Construct: ORF TRCN0000470152
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000150.1_s317c1
- Derived from:
- ccsbBroadEn_02007
- DNA Barcode:
- ATGTGTTGACGTCTTTACATGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM18 (8749)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470152
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NM_014237.3 | 100% | 100% | |
| 2 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NM_001320313.2 | 96.7% | 96.7% | 588_589ins72 |
| 3 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NR_135201.2 | 77.2% | (many diffs) | |
| 4 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NM_001190956.2 | 24.3% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2286
- ORF length:
- 2217
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttccttctc ctcgccctcc tcactgagct tggaagactg caagcccacg 121 aaggttctga aggaatattt ctgcatgtca cagttccacg gaagattaag tcaaatgaca 181 gtgaagtttc agagaggaag atgatttaca tcattacaat tgatggacaa ccttacactc 241 tacatctcgg aaaacaatca ttcttacccc agaacttttt ggtttataca tataatgaaa 301 ctggatcttt gcattctgtg tctccatatt ttatgatgca ttgccattac caaggatatg 361 ctgccgaatt tccaaattca tttgtgacac tcagtatatg ttctggtctc aggggatttc 421 tccagtttga aaatatcagt tatggaattg aaccagtaga atcttcagca agatttgagc 481 atataattta tcaaatgaaa aataatgatc caaatgtatc cattttagca gtaaattaca 541 gtcatatttg gcagaaagac cagccctaca aagttccttt aaactcacag ataaaaaatc 601 tttcaaaact attaccccaa tatctggaaa tatacattat agtggaaaaa gctttgtatg 661 attatatggg atctgaaatg atggctgtaa cacaaaaaat tgtccaggtt attgggcttg 721 tcaacactat gtttacccag ttcaaattga ctgttatact gtcttccttg gaattgtggt 781 caaatgaaaa ccagatttcc accagtgggg atgctgatga tatattacaa agatttttgg 841 catggaaacg ggactatctc atcctacggc cccatgacat agcatactta cttgtttaca 901 ggaaacatcc taaatatgtg ggagcaacat ttcctggcac tgtatgcaat aaaagctatg 961 atgcaggtat tgctatgtat ccagatgcaa taggtttgga gggattttcg gttattatag 1021 ctcaactgct tggccttaat gtaggattaa catatgatga catcactcag tgtttctgtc 1081 tgagagctac atgcatcatg aatcatgaag cagtgagtgc cagtggtaga aagattttta 1141 gcaactgcag catgcacgac tatagatatt ttgtttcaaa atttgagact aaatgccttc 1201 agaagctttc aaatttgcaa ccattacatc aaaatcaacc agtgtgtggt aatgggattt 1261 tggaatccaa tgaagaatgt gactgtggta ataaaaatga atgtcaattt aagaagtgct 1321 gtgattataa cacatgtaaa ctgaagggct cagtaaaatg tggttctgga ccatgttgta 1381 catcaaagtg tgagttgtca atagcaggca ctccatgtag aaagagtatt gatccagagt 1441 gtgattttac agagtactgc aatggaacct ctagtaattg tgttcctgac acttatgcat 1501 tgaatggccg tttgtgcaag ttgggaactg cctattgcta taacggacaa tgtcaaacta 1561 ctgataacca gtgtgccaag atatttggaa aaggtgctca aggtgctcca tttgcctgtt 1621 ttaaagaagt taattctctg catgaaagat ctgaaaactg tggttttaaa aattcacaac 1681 cattaccttg tgaacggaag gatgttctct gtggaaaatt agcttgtgtt cagccacata 1741 aaaatgctaa taaaagtgac gctcaatcta cagtttattc atatattcaa gaccatgtat 1801 gtgtatctat agccactggt tcctccatga gatcagatgg aacagacaat gcctatgtgg 1861 ctgatggcac catgtgtggT CCAGAAATGT ACTGTGTAAA TAAAACCTGC AGAAAAGTTC 1921 ATTTAATGGG ATATAACTGT AATGCCACCA CAAAATGCAA AGGGAAAGGG ATATGTAATA 1981 ATTTTGGTAA TTGTCAATGC TTCCCTGGAC ATAGACCTCC AGATTGTAAA TTCCAGTTTG 2041 GTTCCCCAGG GGGTAGTATT GATGATGGAA ATTTTCAGAA ATCTGGTGAC TTTTATACTG 2101 AAAAAGGCTA CAATACACAC TGGAACAACT GGTTTATTCT GAGTTTCTGC ATTTTTCTGC 2161 CGTTTTTCAT AGTTTTCACC ACTGTGATCT TTAAAAGAAA TGAAATAAGT AAATCATGTA 2221 ACAGAGAGAA TGCAGAGTAT AATCGTAATT CATCCGTTGT ATCAGAAAGC GATGACGTGG 2281 GACATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2341 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2401 CTTGGCTTTA ATATATCTTG TGGAAAGGAC GAATGTGTTG ACGTCTTTAC ATGCTGACGC 2461 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt