Construct: ORF TRCN0000470157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016406.1_s317c1
- Derived from:
- ccsbBroadEn_05680
- DNA Barcode:
- GCCGGTAGGCGTGTGCACGGCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTL8B (441518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470157
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441518 | RTL8B | retrotransposon Gag like 8B | NM_001078173.2 | 100% | 100% | |
| 2 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001078172.1 | 96.4% | 96.4% | (many diffs) |
| 3 | human | 8933 | RTL8C | retrotransposon Gag like 8C | NM_001078171.2 | 94.3% | 92.9% | (many diffs) |
| 4 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001134321.2 | 64.5% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 432
- ORF length:
- 339
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGAAGG TCGAGTGCAG CTGATGAAGG 121 CCCTCCTGGC TCGGCCCCTC CGGCCCGCGG CGCGTCGCTG GAGGAACCCG ATTCCCTTTC 181 CCGAGACGTT TGATGGCGAT ACCGACCGGC TCCCGGAGTT CATCGTGCAG ACGAGCTCCT 241 ACATGTTCGT GGACGAGAAC ACGTTCTCCA ACGACGCCCT GAAGGTGACG TTCCTCATCA 301 CCCGCCTCAC GGGGCCCGCC CTGCAGTGGG TGATCCCCTA CATCAAGAAG GAGAGCCCCC 361 TCCTCAGTGA TTACCGGGGC TTCCTGGCTG AGATGAAGCG GGTCTTTGGA TGGGAGGAGG 421 ACGAGGACTT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC GGTAGGCGTG TGCACGGCCC 601 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t