Construct: ORF TRCN0000470164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009134.1_s317c1
- Derived from:
- ccsbBroadEn_05065
- DNA Barcode:
- CGACGTCACAGCGTCCTCGGTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPICE1 (152185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470164
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152185 | SPICE1 | spindle and centriole assoc... | NM_001331078.2 | 100% | 100% | |
| 2 | human | 152185 | SPICE1 | spindle and centriole assoc... | NM_144718.4 | 100% | 100% | |
| 3 | human | 152185 | SPICE1 | spindle and centriole assoc... | NM_001331079.1 | 98.3% | 98.3% | 1_42del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2631
- ORF length:
- 2565
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atttgtcaga gtgaaccgct gtggtccccg agttggtgta agaaagacac 121 cgaaagtaaa gaagaagaaa acttcagtga aacaagaatg ggataatacc gtgactgatc 181 taaccgttca tcgggcaact cccgaagatc tggtacgccg tcatgaaata cacaaatcga 241 agaatagagc attagtacac tgggaactcc aagaaaaagc tttgaagaga aaatggagga 301 agcagaaacc agaaacttta aatcttgaga aaagaagatt gtctatcatg aaggagattc 361 tttctgatca ataccagatg caagatgtgt tggagaaatc tgatcatcta atagctgcag 421 caaaagagct gtttcctcgt aggcgcacag ggtttccaaa tgtaacagtg gctcctgatt 481 cctctcaggg tcccattgtg gtaaatcaag accctatcac ccaatctatc tttaatgagt 541 ctgtcataga acctcaggct cttaatgatg tagatggtga agaagaagga actgttaata 601 gccagtcagg agaaagtgag aatgagaatg agttggataa ctctctaaac tctcagtcta 661 acacgaatac agacaggttt ctccaacaac taacagaaga gaattttgag ttaattagta 721 agttgtggac tgacattcag cagaaaatag caacccagtc acaaataact cctccaggaa 781 cgccatcatc tgctctttca tcaggggagc aaagagctgc tctgaatgct accaatgctg 841 tcaagagact ccaaaccagg cttcagcctg aagaatctac tgagactcta gactcaagct 901 acgttgtggg acacgtgctg aactcaagga agcaaaaaca gctgttaaat aaagtgaaaa 961 ggaaaccgaa tttgcatgct ctttccaagc cgaagaaaaa catatcatca ggtagcacaa 1021 cctctgcaga cttaccaaat aggactaatt ccaacctgga tgtcctcaaa cacatgatac 1081 atgaagtgga acatgaaatg gaagaatatg agcggtggac aggtcgcgag gtcaagggtc 1141 tgcagagcag tcagggtctt acaggcttca ctttgtcgct ggtgagctcc ctctgtcgcc 1201 tggttcggta ccttaaagag agtgagatcc agctacgtaa agaagtagag acaaggcaac 1261 aactggaaca agtattaggt gatcatcgag agctcattga tgctctgaca gctgaaattc 1321 ttcgtcttag agaagaaaac gctgctacac aggcaagact tcagcagtac atggtcacaa 1381 cagatgagca actgatatca ctcacacatg ctattaagaa ctgtcctgtg ataaataaca 1441 gacaagaaat tcaggcatca gaaagcggag ccacaggtag aagagttatg gacagtccag 1501 agcgtccagt tgtaaatgcc aatgtctcag tgccattgat gttcagagag gaagtggctg 1561 aattcccaca ggaagagttg cccgttaaac tgtctcaggt gccagaccct ccagataaca 1621 tgaatctggc caagaatttt ccagcacata tttttgagcc agctgtgttg ttaacaccac 1681 ccaggcagaa gagcaactta aaattctctc ctcttcagga cgtattgaga aggactgttc 1741 aaactcgtcc tgctccacga cttcctccaa ctgtggaaat aattgagaag gaacaaaatt 1801 gggaagagaa gaccttacct attgatacag acattcagaa ttcaagtgaa gagaatcgtc 1861 tcttcactca gagatggaga gtctctcaca tgggagaaga tttggagaac aaaactcagg 1921 ctccttttgt taacctctca cagcccctct gcaattccca ttccaacact caacagtcaa 1981 gaagccccac attctcagaa gagctcccag tactgggaga tgggcagcag ctgagaacaa 2041 atgagtcatt aatacaaaga aaggacataa tgacacgaat tgctgatttg acattgcaga 2101 attcagctat caaggcacat atgaataata ttattgagcc cagaggagag caaggggatg 2161 gacTCCGGGA GTTGAACAAA CAAGAAAGTG CAAGTGACAT GACTTCTACT TTTCCAGTAG 2221 CACAGTCTCT AACACCAGGT AGTATGGAGG AACGGATTGC AGAATTGAAT CGACAAAGTA 2281 TGGAGGCTCG TGGAAAACTA CTGCAGTTGA TAGAGCAGCA GAAACTTGTT GGTTTGAATC 2341 TTTCTCCACC AATGTCACCT GTTCAGTTAC CTCTCAGAGC ATGGACTGAA GGAGCAAAGA 2401 GGACAATTGA GGTATCTATT CCAGGAGCAG AAGCCCCAGA AAGCTCAAAA TGTAGTACTG 2461 TCTCTCCCGT CAGCGGGATA AATACAAGAA GATCTTCCGG GGCTACTGGT AATTCTTGTT 2521 CTCCACTAAA TGCCACCTCA GGAAGTGGGA GATTCACACC TCTTAATCCA AGAGCAAAGA 2581 TTGAGAAACA GAATGAAGAA GGCTGGTTTG CTCTTTCTAC CCATGTATCA TACCCAACTT 2641 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2701 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2761 CTTGTGGAAA GGACGACGAC GTCACAGCGT CCTCGGTTCG ACGCGTTAAG TCgacaatca 2821 acctctggat tacaaaattt gtgaaagatt