Construct: ORF TRCN0000470167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000479.1_s317c1
- Derived from:
- ccsbBroadEn_07275
- DNA Barcode:
- AGACGGCTTAACTCGTTCTTCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMG1 (8624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8624 | PSMG1 | proteasome assembly chapero... | NM_003720.4 | 99.7% | 100% | 687A>G;849G>A |
| 2 | human | 8624 | PSMG1 | proteasome assembly chapero... | NM_001261824.1 | 98% | 98.2% | 392_393insGGTTTTTCTCTGTCA;672A>G;834G>A |
| 3 | human | 8624 | PSMG1 | proteasome assembly chapero... | NM_203433.2 | 92.4% | 92.7% | 392_393ins63;624A>G;786G>A |
| 4 | human | 8624 | PSMG1 | proteasome assembly chapero... | NM_001320795.1 | 69.5% | 69.7% | 0_1ins261;426A>G;588G>A |
| 5 | human | 8624 | PSMG1 | proteasome assembly chapero... | XM_024452135.1 | 69.5% | 69.7% | 0_1ins261;426A>G;588G>A |
| 6 | human | 8624 | PSMG1 | proteasome assembly chapero... | NR_049728.1 | 66.2% | (many diffs) | |
| 7 | mouse | 56088 | Psmg1 | proteasome (prosome, macrop... | NM_019537.3 | 80.9% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccacgttc ttcggagagg tggtgaaggc gccgtgccga gctgggactg 121 aggacgaaga ggaggaggag gaggggcgga gggagacgcc cgaggacagg gaggtgcgtc 181 tgcagctggc gcggaagagg gaagtgcggc tccttcgaag acaaacaaaa acatctttgg 241 aagtttcttt gctagaaaaa tatccgtgct ccaagtttat aattgctata ggaaataatg 301 cagtagcatt tctgtcatca tttgttatga attcaggagt ctgggaggaa gttggttgtg 361 ctaaactctg gaatgaatgg tgtagaacaa cagacactac acatctgtcc tccacagagg 421 ctttttgtgt gttttatcat ctaaaatcca atCCCTCGGT TTTTCTCTGT CAGTGCAGTT 481 GCTATGTTGC AGAAGATCAA CAGTATCAGT GGCTGGAAAA GGTTTTTGGC TCTTGTCCAA 541 GGAAGAACAT GCAGATAACT ATTCTCACAT GTCGACATGT TACCGATTAT AAAACCTCAG 601 AATCCACCGG CAGCCTTCCT TCTCCTTTCC TGAGAGCCCT AAAAACACAG AATTTCAAAG 661 ACTCGGCGTG TTGTCCATTG CTAGAACAAC CGAATATAGT ACACGACCTT CCTGCAGCAG 721 TTCTAAGCTA CTGTCAAGTA TGGAAAATCC CGGCAATTCT GTACTTGTGT TATACTGATG 781 TGATGAAATT AGACCTAATC ACAGTGGAAG CTTTTAAGCC TATACTTTCT ACCAGAAGCT 841 TGAAGGGTTT GGTTAAGAAT ATTCCCCAAA GCACTGAGAT ACTAAAGAAA TTGATGACAA 901 CAAATGAGAT TCAAAGTAAC ATTTATACAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGACG 1081 GCTTAACTCG TTCTTCCACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt