Construct: ORF TRCN0000470189
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008664.1_s317c1
- Derived from:
- ccsbBroadEn_16169
- DNA Barcode:
- ATCGGAGGTTAAGTGCCTATTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MZT2A (653784)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470189
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653784 | MZT2A | mitotic spindle organizing ... | NM_001085365.2 | 76.5% | 76.5% | 1_111del |
| 2 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_025029.5 | 74.6% | 72.7% | (many diffs) |
| 3 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453048.1 | 57.8% | 57.8% | 1_264del |
| 4 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453049.1 | 57.8% | 57.8% | 1_264del |
| 5 | human | 80097 | MZT2B | mitotic spindle organizing ... | NM_001330282.2 | 54.1% | 52.7% | (many diffs) |
| 6 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453051.1 | 44.9% | 44.9% | 1_264del;471_650del |
| 7 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_005263742.3 | 41.9% | 34.4% | (many diffs) |
| 8 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_017004744.2 | 41.6% | 34.7% | (many diffs) |
| 9 | human | 653784 | MZT2A | mitotic spindle organizing ... | XM_024453050.1 | 41.6% | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgtacgag ctggctcagg cggcgggcgg cggtatcgac cccgacgtgt 121 tcaagatcct ggtggacctg ctGAAGCTGA ACGTGGCCCC CCTCGCCGTC TTCCAGATGC 181 TCAAGTCCAT GTGTGCCGGG CAGAGGCTAG CGAGCGAGCC CCAGGACCCT GCGGCCGTGT 241 CTCTGCCCAC GTCGAGCGTG CCCGAGACCC GAGGGAGAGA CAAAGGCAGC GCTGCCCTCG 301 GGGGAGTATT GGCCCTGGCG GAACGCAGCA ACCACGAGGG ATCCAGCCAG AGGATGCCAC 361 GCCAGCCCAG CGCTACCAGG CTGCCCAAGG GGGGCGGGCC TGGGAAGAGC CCTACGCAGG 421 GCAGCACCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCGGA GGTTAAGTGC CTATTGCCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt