Construct: ORF TRCN0000470192
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007250.1_s317c1
- Derived from:
- ccsbBroadEn_04008
- DNA Barcode:
- ACCGACACCCACACTTTTCTGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM108 (66000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470192
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 66000 | TMEM108 | transmembrane protein 108 | NM_001136469.2 | 100% | 100% | |
| 2 | human | 66000 | TMEM108 | transmembrane protein 108 | NM_023943.4 | 100% | 100% | |
| 3 | human | 66000 | TMEM108 | transmembrane protein 108 | NM_001282865.1 | 17.3% | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagaagttta caggccctct attgccaact gttaagtttc ctgctgatct 121 tggcactgac cgaagcgctg gcatttgcca tccaggaacc atctcccagg gaatctcttc 181 aggtcctccc ttcaggcact cccccgggaa ccatggtgac agcaccccac agctctacca 241 gacatacttc tgtggtgatg ctgaccccca atcccgatgg acccccctca caggctgcag 301 ctcccatggc aacaccgaca ccccgtgcag aggggcaccc tcctacgcac accatctcca 361 ccatcgctgc gacagtaacc gccccccatt ctgaaagctc cctgtccaca gggcccgctc 421 cagcagccat ggcaaccaca tcctccaagc cagagggccg ccctcgaggg caggctgccc 481 ccaccatcct gctgacaaag ccaccggggg ccaccagccg ccccaccaca gcgccccccc 541 gcactaccac acgcaggccc cccaggcccc caggctcttc ccgaaaaggg gctggtaatt 601 catcacgccc tgtcccgcct gcacctggtg gccactccag gagtaaagaa ggacagcgag 661 gacgaaatcc aagctccaca cctctggggc agaagcggcc cctggggaaa atctttcaga 721 tctacaaggg caacttcaca gggtctgtgg aaccggagcc ctctaccctc acccccagga 781 ccccactctg gggctactcc tcttcaccac agccccagac agtggctgcg accacagtgc 841 ccagcaatac ctcatgggca cccaccacca cctccctggg gcctgcaaag gacaagccag 901 gccttcgcag agcagcccag gggggtggtt ctaccttcac cagccaagga gggacaccag 961 atgccacagc agcctcaggt gcccctgtca gtccacaagc tgccccagtg ccttctcagc 1021 gcccccacca cggtgaccca caggatggcc ccagccatag tgactcttgg cttactgtta 1081 cccctggcac cagcagacct ctgtctacca gctctggggt cttcacggct gccacggggc 1141 ccaccccagc tgccttcgat accagtgtct cagccccttc ccaggggatt cctcagggag 1201 catccacaac cccacaagct ccaacccatc cctccagggt ctcagaaagc actatttctg 1261 gagccaagga ggagactgtg gccaccctca ccatgaccga ccgggtgccc agtcctctct 1321 ccacagtggt atccacagcc acaggcaatt tcctcaaccg cctggtcccc gccgggacct 1381 ggaagcctgg gacagcaggg aacatctccc atgtggccga gggggacaaa ccgcagcaca 1441 gagccaccat ctgcctgagc aagatggata tcgcctgggt gaTCCTGGCC ATCAGCGTGC 1501 CCATCTCCTC CTGCTCTGTC CTGCTGACGG TGTGCTGCAT GAAGAGGAAG AAGAAGACCG 1561 CCAACCCGGA GAACAACCTG AGCTACTGGA ACAACACCAT CACCATGGAC TACTTCAACA 1621 GGCATGCTGT GGAGCTGCCC AGGGAGATCC AGTCCCTTGA AACCTCTGAG GACCAGCTCT 1681 CAGAGCCCCG CTCCCCAGCC AATGGCGACT ATAGAGACAC TGGGATGGTC CTTGTTAACC 1741 CCTTCTGTCA AGAAACACTG TTTGTGGGAA ACGATCAAGT ATCTGAGATC TACCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGAACCG ACACCCACAC TTTTCTGCCT ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt