Construct: ORF TRCN0000470206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014394.1_s317c1
- Derived from:
- ccsbBroadEn_08939
- DNA Barcode:
- CTCTAGACATGACGTTGCTGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf54 (79630)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470206
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79630 | C1orf54 | chromosome 1 open reading f... | NM_001301040.1 | 99.7% | 100% | 303T>C |
| 2 | human | 79630 | C1orf54 | chromosome 1 open reading f... | NM_024579.4 | 99.7% | 100% | 303T>C |
| 3 | human | 79630 | C1orf54 | chromosome 1 open reading f... | XM_024449802.1 | 99.7% | 100% | 303T>C |
| 4 | human | 79630 | C1orf54 | chromosome 1 open reading f... | NM_001301039.1 | 98.9% | 99.2% | 301_303delCAG;306T>C |
| 5 | human | 79630 | C1orf54 | chromosome 1 open reading f... | XM_024449801.1 | 98.9% | 99.2% | 301_303delCAG;306T>C |
| 6 | human | 79630 | C1orf54 | chromosome 1 open reading f... | NM_001301042.1 | 78.8% | 71.5% | (many diffs) |
| 7 | human | 79630 | C1orf54 | chromosome 1 open reading f... | NM_001301041.1 | 77.7% | 78% | 45_46ins84;217_219delCAG;222T>C |
| 8 | human | 79630 | C1orf54 | chromosome 1 open reading f... | XM_011509984.3 | 77.7% | 78% | 45_46ins84;217_219delCAG;222T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgtcctcttt gtagccatct ttgctgtgcc acttatcctg ggacaagaat 121 atgaggatga agaaagactg ggagaggatg aatattatca ggtggtctat tattatacag 181 tcacccccag ttatgatgac tttagtgcag atttcaccat tgattactcc atattTGAGT 241 CAGAGGACAG GCTGAACAGG TTGGATAAGG ACATAACAGA AGCAATAGAG ACTACCATTA 301 GTCTTGAAAC AGCACGTGCA GACCATCCGA AGCCTGTAAC TGTGAAACCA GTAACAACGG 361 AACCTAGCCC AGATCTGAAC GATGCCGTGT CCAGTTTGCG AAGTCCTATT CCCCTCCTCC 421 TGTCGTGTGC CTTTGTTCAG GTGGGGATGT ATTTCATGTA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGACTCTAG ACATGACGTT GCTGGCGCAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt