Transcript: Human NM_001301039.1

Homo sapiens chromosome 1 open reading frame 54 (C1orf54), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-02-24
Taxon:
Homo sapiens (human)
Gene:
C1orf54 (79630)
Length:
641
CDS:
150..548

Additional Resources:

NCBI RefSeq record:
NM_001301039.1
NBCI Gene record:
C1orf54 (79630)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001301039.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263665 TATTATCAGGTGGTCTATTAT pLKO_005 237 CDS 100% 15.000 21.000 N C1orf54 n/a
2 TRCN0000263668 TGATGACTTTAGTGCAGATTT pLKO_005 278 CDS 100% 13.200 18.480 N C1orf54 n/a
3 TRCN0000263667 ACCATTGATTACTCCATATTT pLKO_005 300 CDS 100% 15.000 10.500 N C1orf54 n/a
4 TRCN0000263669 ACAGGTTGGATAAGGACATAA pLKO_005 340 CDS 100% 13.200 9.240 N C1orf54 n/a
5 TRCN0000369988 ACTTATCCTGGGACAAGAATA pLKO_005 185 CDS 100% 13.200 9.240 N C1orf54 n/a
6 TRCN0000377365 AGACCATCCGAAGCCTGTAAC pLKO_005 404 CDS 100% 10.800 7.560 N C1orf54 n/a
7 TRCN0000263666 AGCAATAGAGACTACCATTAG pLKO_005 365 CDS 100% 10.800 7.560 N C1orf54 n/a
8 TRCN0000168180 CACTTATCCTGGGACAAGAAT pLKO.1 184 CDS 100% 5.625 3.938 N C1orf54 n/a
9 TRCN0000167852 GAATATTATCAGGTGGTCTAT pLKO.1 234 CDS 100% 4.950 3.465 N C1orf54 n/a
10 TRCN0000172732 GCCTGTAACTGTGAAACCAGT pLKO.1 416 CDS 100% 2.640 1.848 N C1orf54 n/a
11 TRCN0000377414 ACCAGTAACAACGGAACCTAG pLKO_005 431 CDS 100% 4.050 5.670 N C1orf54 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001301039.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08939 pDONR223 100% 98.9% 99.2% None 301_303delCAG;306T>C n/a
2 ccsbBroad304_08939 pLX_304 0% 98.9% 99.2% V5 301_303delCAG;306T>C n/a
3 TRCN0000470206 CTCTAGACATGACGTTGCTGGCGC pLX_317 56.6% 98.9% 99.2% V5 301_303delCAG;306T>C n/a
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