Construct: ORF TRCN0000470234
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000192.1_s317c1
- Derived from:
- ccsbBroadEn_10891
- DNA Barcode:
- GATCAAGAACGCCAGGATTCAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSF2 (3298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470234
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3298 | HSF2 | heat shock transcription fa... | NM_001243094.2 | 100% | 100% | |
| 2 | human | 3298 | HSF2 | heat shock transcription fa... | NM_001135564.1 | 44.2% | 44% | 684_700del;703_704delGGinsTT;708_1554del |
| 3 | human | 3298 | HSF2 | heat shock transcription fa... | NM_004506.4 | 42.7% | 42.5% | 684_700del;703_704delGGinsTT;708_1608del |
| 4 | mouse | 15500 | Hsf2 | heat shock factor 2 | NM_008297.3 | 41.3% | 43.3% | (many diffs) |
| 5 | mouse | 15500 | Hsf2 | heat shock factor 2 | XM_006512564.2 | 39.9% | 41.8% | (many diffs) |
| 6 | mouse | 15500 | Hsf2 | heat shock factor 2 | XM_006512565.3 | 29.9% | 31.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcagagttcg aacgtgccgg ctttcctcag caagctgtgg acgcttgtgg 121 aggaaaccca cactaacgag ttcatcacct ggagccagaa tggccaaagt tttctggtct 181 tggatgagca acgatttgca aaagaaattc ttcccaaata tttcaagcac aataatatgg 241 caagctttgt gaggcaactg aatatgtatg gtttccgtaa agtagtacat atcgactctg 301 gaattgtaaa gcaagaaaga gatggtcctg tagaatttca gcatccttac ttcaaacaag 361 gacaggatga cttgttggag aacattaaaa ggaaggtttc atcTTCAAAA CCAGAAGAAA 421 ATAAAATTCG TCAGGAAGAT TTAACAAAAA TTATAAGTAG TGCTCAGAAG GTTCAGATAA 481 AACAGGAAAC TATTGAGTCC AGGCTTTCTG AATTAAAAAG TGAGAATGAG TCCCTTTGGA 541 AGGAGGTGTC AGAATTACGA GCAAAGCATG CACAACAGCA ACAAGTTATT CGAAAGATTG 601 TCCAGTTTAT TGTTACATTG GTTCAAAATA ACCAACTTGT GAGTTTAAAA CGTAAAAGGC 661 CTCTACTTCT AAACACTAAT GGAGCCCAAA AGAAGAACCT GTTTCAGCAC ATAGTCAAAG 721 AACCAACTGA TAATCATCAT CATAAAGTAA TTTTTTACCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 AGATCAAGAA CGCCAGGATT CAGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt