Construct: ORF TRCN0000470235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001458.1_s317c1
- Derived from:
- ccsbBroadEn_01723
- DNA Barcode:
- GCTTCGGACGCCATTCTTCGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTK (7272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470235
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7272 | TTK | TTK protein kinase | NM_003318.5 | 100% | 100% | |
| 2 | human | 7272 | TTK | TTK protein kinase | XM_011536099.3 | 100% | 100% | |
| 3 | human | 7272 | TTK | TTK protein kinase | NM_001166691.1 | 99.8% | 99.8% | 1255_1256insAGC |
| 4 | human | 7272 | TTK | TTK protein kinase | XM_011536100.3 | 99.8% | 99.8% | 1255_1256insAGC |
| 5 | human | 7272 | TTK | TTK protein kinase | XM_017011242.2 | 93.5% | 93.5% | 0_1ins165 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2637
- ORF length:
- 2571
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atccgaggat ttaagtggca gagaattgac aattgattcc ataatgaaca 121 aagtgagaga cattaaaaat aagtttaaaa atgaagacct tactgatgaa ctaagcttga 181 ataaaatttc tgctgatact acagataact cgggaactgt taaccaaatt atgatgatgg 241 caaacaaccc agaggactgg ttgagtttgt tgctcaaact agagaaaaac agtgttccgc 301 taagtgatgc tcttttaaat aaattgattg gtcgttacag tcaagcaatt gaagcgcttc 361 ccccagataa atatggccaa aatgagagtt ttgctagaat tcaagtgaga tttgctgaat 421 taaaagctat tcaagagcca gatgatgcac gtgactactt tcaaatggcc agagcaaact 481 gcaagaaatt tgcttttgtt catatatctt ttgcacaatt tgaactgtca caaggtaatg 541 tcaaaaaaag taaacaactt cttcaaaaag ctgtagaacg tggagcagta ccactagaaa 601 tgctggaaat tgccctgcgg aatttaaacc tccaaaaaaa gcagctgctt tcagaggagg 661 aaaagaagaa tttatcagca tctacggtat taactgccca agaatcattt tccggttcac 721 ttgggcattt acagaatagg aacaacagtt gtgattccag aggacagact actaaagcca 781 ggtttttata tggagagaac atgccaccac aagatgcaga aataggttac cggaattcat 841 tgagacaaac taacaaaact aaacagtcat gcccatttgg aagagtccca gttaaccttc 901 taaatagccc agattgtgat gtgaagacag atgattcagt tgtaccttgt tttatgaaaa 961 gacaaacctc tagatcagaa tgccgagatt tggttgtgcc tggatctaaa ccaagtggaa 1021 atgattcctg tgaattaaga aatttaaagt ctgttcaaaa tagtcatttc aaggaacctc 1081 tggtgtcaga tgaaaagagt tctgaactta ttattactga ttcaataacc ctgaagaata 1141 aaacggaatc aagtcttcta gctaaattag aagaaactaa agagtatcaa gaaccagagg 1201 ttccagagag taaccagaaa cagtggcaat ctaagagaaa gtcagagtgt attaaccaga 1261 atcctgctgc atcttcaaat cactggcaga ttccggagtt agcccgaaaa gttaatacag 1321 agcagaaaca taccactttt gagcaacctg tcttttcagt ttcaaaacag tcaccaccaa 1381 tatcaacatc taaatggttt gacccaaaat ctatttgtaa gacaccaagc agcaatacct 1441 tggatgatta catgagctgt tttagaactc cagttgtaaa gaatgacttt ccacctgctt 1501 gtcagttgtc aacaccttat ggccaacctg cctgtttcca gcagcaacag catcaaatac 1561 ttgccactcc acttcaaaat ttacaggttt tagcatcttc ttcagcaaat gaatgcattt 1621 cggttaaagg aagaatttat tccatattaa agcagatagg aagtggaggt tcaagcaagg 1681 tatttcaggt gttaaatgaa aagaaacaga tatatgctat aaaatatgtg aacttagaag 1741 aagcagataa ccaaactctt gatagttacc ggaacgaaat agcttatttg aataaactac 1801 aacaacacag tgataagatc atccgacttt atgattatga aatcacggac cagtacatct 1861 acatggtaat ggagtgtgga aatattgatc ttaatagttg gcttaaaaag aaaaaatcca 1921 ttgatccatg ggaacgcaag agttactgga aaaatatgtt agaggcagtt cacacaatcc 1981 atcaacatgg cattgttcac agtgatctta aaccagctaa ctttctgata gttgatggaa 2041 tgctaaagct aattgatttt gggattgcaa accaaatgca accagataca acaagtgttg 2101 ttaaagattc tcaggttggc acagttaatt atatgccacc agaagcaatc aaagatatgt 2161 cttcctccag agagaatggg aaatcTAAGT CAAAGATAAG CCCCAAAAGT GATGTTTGGT 2221 CCTTAGGATG TATTTTGTAC TATATGACTT ACGGGAAAAC ACCATTTCAG CAGATAATTA 2281 ATCAGATTTC TAAATTACAT GCCATAATTG ATCCTAATCA TGAAATTGAA TTTCCCGATA 2341 TTCCAGAGAA AGATCTTCAA GATGTGTTAA AGTGTTGTTT AAAAAGGGAC CCAAAACAGA 2401 GGATATCCAT TCCTGAGCTC CTGGCTCATC CATATGTTCA AATTCAAACT CATCCAGTTA 2461 ACCAAATGGC CAAGGGAACC ACTGAAGAAA TGAAATATGT TCTGGGCCAA CTTGTTGGTC 2521 TGAATTCTCC TAACTCCATT TTGAAAGCTG CTAAAACTTT ATATGAACAC TATAGTGGTG 2581 GTGAAAGTCA TAATTCTTCA TCCTCCAAGA CTTTTGAAAA AAAAAGGGGA AAAAAATGCC 2641 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2701 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2761 ATATATCTTG TGGAAAGGAC GAGCTTCGGA CGCCATTCTT CGATTCACGC GTTAAGTCga 2821 caatcaacct ctggattaca aaatttgtga aagatt