Construct: ORF TRCN0000470243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014533.1_s317c1
- Derived from:
- ccsbBroadEn_06190
- DNA Barcode:
- TATTTTTTGACTCGGTCAAAGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FABP5 (2171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2171 | FABP5 | fatty acid binding protein 5 | NM_001444.3 | 99.7% | 99.2% | 13C>G |
| 2 | human | 220832 | FABP5P3 | fatty acid binding protein ... | NR_002935.1 | 45.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 495
- ORF length:
- 405
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCACAGT TGAGCAGCTG GAAGGAAGAT 121 GGCGCCTGGT GGACAGCAAA GGCTTTGATG AATACATGAA GGAGCTAGGA GTGGGAATAG 181 CTTTGCGAAA AATGGGCGCA ATGGCCAAGC CAGATTGTAT CATCACTTGT GATGGTAAAA 241 ACCTCACCAT AAAAACTGAG AGCACTTTGA AAACAACACA GTTTTCTTGT ACCCTGGGAG 301 AGAAGTTTGA AGAAACCACA GCTGATGGCA GAAAAACTCA GACTGTCTGC AACTTTACAG 361 ATGGTGCATT GGTTCAGCAT CAGGAGTGGG ATGGGAAGGA AAGCACAATA ACAAGAAAAT 421 TGAAAGATGG GAAATTAGTG GTGGAGTGTG TCATGAACAA TGTCACCTGT ACTCGGATCT 481 ATGAAAAAGT AGAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATTTTTTGA CTCGGTCAAA 661 GTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt