Construct: ORF TRCN0000470250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005618.1_s317c1
- Derived from:
- ccsbBroadEn_12990
- DNA Barcode:
- ACAAGGCCCCGGGGTCAATTCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNG4 (93107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93107 | KCNG4 | potassium voltage-gated cha... | NM_172347.2 | 49% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc catgccttcc agagacgggg gcctgcatcc cagacaccac cactatggtt 121 cccacagccc ttggagtcag ctcctgtcca gccccatgga gacgccgtcc atcaagggcc 181 tttactaccg gagggtgcgg aaggtgggtg ccctggacgc ctccccagtg gacctgaaga 241 aggagatcct gatcaacgtg gggggcagga ggtatctcct cccctggagc acactggacc 301 ggttcccgct gagccgcctg agcaaactca ggctctgtcg gagctacgag gagatcgtgc 361 agctctgcga tgattacgaC GAGGACAGCC AGGAGTTCTT CTTCGACAGG AGCCCCAGCG 421 CCTTCGGGGT GATCGTGAGC TTCCTGGCGG CCGGGAAGCT GGTGCTTCTG CAGGAGATGT 481 GCGCGCTGTC CTTCCAGGAG GAGCTGGCCT ACTGGGGCAT CGAGGAGGCC CACCTGGAGA 541 GGTGCTGCCT GCGGAAGCTG CTGAGGAAGC TGGAGGAGCT GGAGGAGCTG GCCAAGCTGC 601 ACAGGGAGGA CGTACTGAGG CAGCAGAGGG AGACCCGCCG CCCCGCCTCG CACTCCTCGC 661 GCTGGGGCCT GTGCATGAAC CGGCTGCGCG AGATGGTGGA AAACCCGCAG TCCGGGCTGC 721 CCGGGAAGGT CTTCGCTTGC CTCTCCATCC TCTTCGTGGC CACCACAGCC GTCAGCCTGT 781 GTGTCAGCAC CATGCCCGAC CTCAGGGCAG AGGAGGACCA GGTGAGCGGC CTCTGCCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAA CAAGGCCCCG GGGTCAATTC AAAACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att