Construct: ORF TRCN0000470258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008139.1_s317c1
- Derived from:
- ccsbBroadEn_04232
- DNA Barcode:
- GACTCACTTTCCCAACCTTTCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2M (81488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81488 | POLR2M | RNA polymerase II subunit M | NM_015532.5 | 100% | 100% | |
| 2 | human | 81488 | POLR2M | RNA polymerase II subunit M | NM_001018102.2 | 57.3% | 57.3% | 113_114ins471 |
| 3 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NM_001285900.3 | 46.5% | 43.5% | (many diffs) |
| 4 | human | 145781 | GCOM1 | GRINL1A complex locus 1 | NR_104371.3 | 17.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1170
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ctcgctgccc cgcggcttcg agccccaagc tcccgaggac ttggcgcagc 121 ggagtttggt ggagctgcgg gaaatgttga agcgccagga gagacttttg cgcaacgaaa 181 aattcatttg caaattgccc gacaaaggta aaaagatctt tgactctttt gccaaactga 241 aagctgccat tgcagaatgt gaagaagtta gaagaaaaag tgaactgttt aaccctgtta 301 gtttagactg taagctaagg caaaaagcaa ttgcagaagt tgatgtgggt acagataagg 361 cccagaattc tgacccgata cttgatactt catcactagt tcctggatgt tcctctgtag 421 ataacatcaa gtcatctcaa acctcacaaa accagggact tggacgtcct actcttgaag 481 gtgatgaaga gacttcagag gttgagtaca cagtgaataa gggcccagct tccagcaata 541 gagacagggt accaccttca tctgaagcta gtgagcatca cccgcggcat cgtgtttcaa 601 gtcaagcgga agatacttcc agcagctttg acaacctgtt tattgacagg ttacagagga 661 tcaccattgc ggaccaaggt gaacaacagt cagaagaaaa cgcaagtact aagaacttGA 721 CAGGCCTTTC CAGTGGGACT GAGAAGAAAC CTCATTACAT GGAAGTGCTA GAAATGCGAG 781 CCAAAAACCC AGTGCCCCAG CTGCGTAAAT TTAAAACCAA TGTGTTACCT TTTCGACAAA 841 ATGATTCATC TAGTCATTGC CAGAAGAGTG GGTCTCCTAT TTCCTCAGAA GAGCGGCGGC 901 GCAGGGATAA GCAGCATCTT GATGACATCA CAGCAGCTCG GCTTCTACCA CTTCACCATA 961 TGCCCACGCA GCTGCTCTCC ATAGAAGAAT CCTTGGCACT TCAGAAACAG CAGAAACAGA 1021 ATTATGAGGA GATGCAAGCA AAGCTCGCAG CGCAAAAATT AGCTGAAAGA CTGAATATTA 1081 AAATGCGGAG TTATAATCCA GAAGGGGAGT CTTCAGGGAG ATACCGAGAA GTAAGGGATG 1141 AAGATGACGA TTGGTCCTCT GATGAATTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1201 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1261 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACTC 1321 ACTTTCCCAA CCTTTCTCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1381 tgaaagatt