Construct: ORF TRCN0000470297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016408.1_s317c1
- Derived from:
- ccsbBroadEn_00344
- DNA Barcode:
- GGATAGTCATGAAAAACCCTGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COMP (1311)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470297
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1311 | COMP | cartilage oligomeric matrix... | NM_000095.3 | 100% | 100% | |
| 2 | mouse | 12845 | Comp | cartilage oligomeric matrix... | NM_016685.2 | 84.5% | 90.8% | (many diffs) |
| 3 | mouse | 12845 | Comp | cartilage oligomeric matrix... | XM_006509539.1 | 72.4% | 78% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2340
- ORF length:
- 2271
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtccccgac accgcctgcg ttcttctgct caccctggct gccctcggcg 121 cgtccggaca gggccagagc ccgttgggct cagacctggg cccgcagatg cttcgggaac 181 tgcaggaaac caacgcggcg ctgcaggacg tgcgggagct gctgcggcag caggtcaggg 241 agatcacgtt cctgaaaaac acggtgatgg agtgtgacgc gtgcgggatg cagcagtcag 301 tacgcaccgg cctacccagc gtgcggcccc tgctccactg cgcgcccggc ttctgcttcc 361 ccggcgtggc ctgcatccag acggagagcg gcgcgcgctg cggcccctgc cccgcgggct 421 tcacgggcaa cggctcgcac tgcaccgacg tcaacgagtg caacgcccac ccctgcttcc 481 cccgagtccg ctgtatcaac accagcccgg ggttccgctg cgaggcttgc ccgccggggt 541 acagcggccc cacccaccag ggcgtggggc tggctttcgc caaggccaac aagcaggttt 601 gcacggacat caacgagtgt gagaccgggc aacataactg cgtccccaac tccgtgtgca 661 tcaacacccg gggctccttc cagtgcggcc cgtgccagcc cggcttcgtg ggcgaccagg 721 cgtccggctg ccagcggcgc gcacagcgct tctgccccga cggctcgccc agcgagtgcc 781 acgagcatgc agactgcgtc ctagagcgcg atggctcgcg gtcgtgcgtg tgtgccgttg 841 gctgggccgg caacgggatc ctctgtggtc gcgacactga cctagacggc ttcccggacg 901 agaagctgcg ctgcccggag cgccagtgcc gtaaggacaa ctgcgtgact gtgcccaact 961 cagggcagga ggatgtggac cgcgatggca tcggagacgc ctgcgatccg gatgccgacg 1021 gggacggggt ccccaatgaa aaggacaact gcccgctggt gcggaaccca gaccagcgca 1081 acacggacga ggacaagtgg ggcgatgcgt gcgacaactg ccggtcccag aagaacgacg 1141 accaaaagga cacagaccag gacggccggg gcgatgcgtg cgacgacgac atcgacggcg 1201 accggatccg caaccaggcc gacaactgcc ctagggtacc caactcagac cagaaggaca 1261 gtgatggcga tggtataggg gatgcctgtg acaactgtcc ccagaagagc aacccggatc 1321 aggcggatgt ggaccacgac tttgtgggag atgcttgtga cagcgatcaa gaccaggatg 1381 gagacggaca tcaggactct cgggacaact gtcccacggt gcctaacagt gcccaggagg 1441 actcagacca cgatggccag ggtgatgcct gcgacgacga cgacgacaat gacggagtcc 1501 ctgacagtcg ggacaactgc cgcctggtgc ctaaccccgg ccaggaggac gcggacaggg 1561 acggcgtggg cgacgtgtgc caggacgact ttgatgcaga caaggtggta gacaagatcg 1621 acgtgtgtcc ggagaacgct gaagtcacgc tcaccgactt cagggccttc cagacagtcg 1681 tgctggaccc ggagggtgac gcgcagattg accccaactg ggtggtgctc aaccagggaa 1741 gggagatcgt gcagacaatg aacagcgacc caggcctggc tgtgggttac actgccttca 1801 atggcgtgga cttcgagggc acgttccatg tgaacacggt cacggatgac gactatgcgg 1861 gcttcatctt tggctaccag gacagctcca gcttctacgt ggtcatgtgg aagcagatgg 1921 agcaaacgta ttggcaggcg aaccccttcc gtgctgtggc cgagcctggc aTCCAACTCA 1981 AGGCTGTGAA GTCTTCCACA GGCCCCGGGG AACAGCTGCG GAACGCTCTG TGGCATACAG 2041 GAGACACAGA GTCCCAGGTG CGGCTGCTGT GGAAGGACCC GCGAAACGTG GGTTGGAAGG 2101 ACAAGAAGTC CTATCGTTGG TTCCTGCAGC ACCGGCCCCA AGTGGGCTAC ATCAGGGTGC 2161 GATTCTATGA GGGCCCTGAG CTGGTGGCCG ACAGCAACGT GGTCTTGGAC ACAACCATGC 2221 GGGGTGGCCG CCTGGGGGTC TTCTGCTTCT CCCAGGAGAA CATCATCTGG GCCAACCTGC 2281 GTTACCGCTG CAATGACACC ATCCCAGAGG ACTATGAGAC CCATCAGCTG CGGCAAGCCT 2341 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2401 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2461 TTTATATATC TTGTGGAAAG GACGAGGATA GTCATGAAAA ACCCTGTTAA CGCGTTAAGT 2521 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt