Construct: ORF TRCN0000470297

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016408.1_s317c1
Derived from:
ccsbBroadEn_00344
DNA Barcode:
GGATAGTCATGAAAAACCCTGTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COMP (1311)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470297
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1311 COMP cartilage oligomeric matrix... NM_000095.3 100% 100%
2 mouse 12845 Comp cartilage oligomeric matrix... NM_016685.2 84.5% 90.8% (many diffs)
3 mouse 12845 Comp cartilage oligomeric matrix... XM_006509539.1 72.4% 78% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2340
ORF length:
2271
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtccccgac accgcctgcg ttcttctgct caccctggct gccctcggcg
121cgtccggaca gggccagagc ccgttgggct cagacctggg cccgcagatg cttcgggaac
181tgcaggaaac caacgcggcg ctgcaggacg tgcgggagct gctgcggcag caggtcaggg
241agatcacgtt cctgaaaaac acggtgatgg agtgtgacgc gtgcgggatg cagcagtcag
301tacgcaccgg cctacccagc gtgcggcccc tgctccactg cgcgcccggc ttctgcttcc
361ccggcgtggc ctgcatccag acggagagcg gcgcgcgctg cggcccctgc cccgcgggct
421tcacgggcaa cggctcgcac tgcaccgacg tcaacgagtg caacgcccac ccctgcttcc
481cccgagtccg ctgtatcaac accagcccgg ggttccgctg cgaggcttgc ccgccggggt
541acagcggccc cacccaccag ggcgtggggc tggctttcgc caaggccaac aagcaggttt
601gcacggacat caacgagtgt gagaccgggc aacataactg cgtccccaac tccgtgtgca
661tcaacacccg gggctccttc cagtgcggcc cgtgccagcc cggcttcgtg ggcgaccagg
721cgtccggctg ccagcggcgc gcacagcgct tctgccccga cggctcgccc agcgagtgcc
781acgagcatgc agactgcgtc ctagagcgcg atggctcgcg gtcgtgcgtg tgtgccgttg
841gctgggccgg caacgggatc ctctgtggtc gcgacactga cctagacggc ttcccggacg
901agaagctgcg ctgcccggag cgccagtgcc gtaaggacaa ctgcgtgact gtgcccaact
961cagggcagga ggatgtggac cgcgatggca tcggagacgc ctgcgatccg gatgccgacg
1021gggacggggt ccccaatgaa aaggacaact gcccgctggt gcggaaccca gaccagcgca
1081acacggacga ggacaagtgg ggcgatgcgt gcgacaactg ccggtcccag aagaacgacg
1141accaaaagga cacagaccag gacggccggg gcgatgcgtg cgacgacgac atcgacggcg
1201accggatccg caaccaggcc gacaactgcc ctagggtacc caactcagac cagaaggaca
1261gtgatggcga tggtataggg gatgcctgtg acaactgtcc ccagaagagc aacccggatc
1321aggcggatgt ggaccacgac tttgtgggag atgcttgtga cagcgatcaa gaccaggatg
1381gagacggaca tcaggactct cgggacaact gtcccacggt gcctaacagt gcccaggagg
1441actcagacca cgatggccag ggtgatgcct gcgacgacga cgacgacaat gacggagtcc
1501ctgacagtcg ggacaactgc cgcctggtgc ctaaccccgg ccaggaggac gcggacaggg
1561acggcgtggg cgacgtgtgc caggacgact ttgatgcaga caaggtggta gacaagatcg
1621acgtgtgtcc ggagaacgct gaagtcacgc tcaccgactt cagggccttc cagacagtcg
1681tgctggaccc ggagggtgac gcgcagattg accccaactg ggtggtgctc aaccagggaa
1741gggagatcgt gcagacaatg aacagcgacc caggcctggc tgtgggttac actgccttca
1801atggcgtgga cttcgagggc acgttccatg tgaacacggt cacggatgac gactatgcgg
1861gcttcatctt tggctaccag gacagctcca gcttctacgt ggtcatgtgg aagcagatgg
1921agcaaacgta ttggcaggcg aaccccttcc gtgctgtggc cgagcctggc aTCCAACTCA
1981AGGCTGTGAA GTCTTCCACA GGCCCCGGGG AACAGCTGCG GAACGCTCTG TGGCATACAG
2041GAGACACAGA GTCCCAGGTG CGGCTGCTGT GGAAGGACCC GCGAAACGTG GGTTGGAAGG
2101ACAAGAAGTC CTATCGTTGG TTCCTGCAGC ACCGGCCCCA AGTGGGCTAC ATCAGGGTGC
2161GATTCTATGA GGGCCCTGAG CTGGTGGCCG ACAGCAACGT GGTCTTGGAC ACAACCATGC
2221GGGGTGGCCG CCTGGGGGTC TTCTGCTTCT CCCAGGAGAA CATCATCTGG GCCAACCTGC
2281GTTACCGCTG CAATGACACC ATCCCAGAGG ACTATGAGAC CCATCAGCTG CGGCAAGCCT
2341TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2401GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2461TTTATATATC TTGTGGAAAG GACGAGGATA GTCATGAAAA ACCCTGTTAA CGCGTTAAGT
2521Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)