Construct: ORF TRCN0000470298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014510.1_s317c1
- Derived from:
- ccsbBroadEn_09353
- DNA Barcode:
- AAATGAAGATTTCGAAACTTTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNG4 (93107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93107 | KCNG4 | potassium voltage-gated cha... | NM_172347.2 | 99.8% | 99.6% | 961G>A;1280G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1626
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccatgcct tccagagacg ggggcctgca tcccagacac caccactatg 121 gttcccacag cccttggagt cagctcctgt ccagccccat ggagacgccg tccatcaagg 181 gcctttacta ccggagggtg cggaaggtgg gtgccctgga cgcctcccca gtggacctga 241 agaaggagat cctgatcaac gtggggggca ggaggtatct cctcccctgg agcacactgg 301 accggttccc gctgagccgc ctgagcaaac tcaggctctg tcggagctac gaggagatcg 361 tgcagctctg cgatgattac gacgaggaca gccaggagtt cttcttcgac aggagcccca 421 gcgccttcgg ggtgatcgtg agcttcctgg cggccgggaa gctggtgctt ctgcaggaga 481 tgtgcgcgct gtccttccag gaggagctgg cctactgggg catcgaggag gcccacctgg 541 agaggtgctg cctgcggaag ctgctgagga agctggagga gctggaggag ctggccaagc 601 tgcacaggga ggacgtactg aggcagcaga gggagacccg ccgccccgcc tcgcactcct 661 cgcgctgggg cctgtgcatg aaccggctgc gcgagatggt ggaaaacccg cagtccgggc 721 tgcccgggaa ggtcttcgct tgcctctcca tcctcttcgt ggccaccaca gccgtcagcc 781 tgtgtgtcag caccatgccc gacctcaggg cagaggagga ccagggcgaa tgctctcgga 841 agtgctacta tattttcatc gtggagacca tctgcgtggc ctggttctcc ctggagttct 901 gcctgcggtt tgtccaggcc caagacaagt gtcagttctt ccaggggccc ctgaacatca 961 tcgacatcct ggccatctcc ccatactacg tgtcgctggc ggtgtctgag gagcccccgg 1021 aggacggcaa gaggccgagc gggagctcct acctggagaa ggtggggctg gtcctgcgtg 1081 tgctgcgagc gctgcgcatc ctctacgtga tgcgcctggc tcgccactcg ctggggctgc 1141 agacgctggg gctcaccgtg cgccgttgca cacgtgagtt cggccTGCTC CTTCTCTTCC 1201 TGGCCGTGGC CATCACCCTC TTCTCCCCTT TGGTCTACGT GGCCGAGAAG GAGTCCGGGC 1261 GGGTGCTGGA GTTCACCAGC ATCCCCGCCT CCTATTGGTG GGCCATCATC TCCATGACAA 1321 CGGTGGGCTA CGGGGACATG GTGCCCCACA GTGTGCCAGG CCAGATGGTG GCCCTCAGCA 1381 GCATCCTGAG CGGGATCCTC ATCATGGCCT TCCCGGCCAC GTCTATCTTC CACACCTTCT 1441 CCCACTCCTA CCTGGAGCTC AAGAAGGAGC AGGAGCAGCT TCAGGCCCGC CTCCGCCACC 1501 TCCAAAACAC CGGTCCAGCC AGTGAATGTG AACTCCTGGA CCCCCATGTG GCCAGTGAAC 1561 ATGAGCTCAT GAACGATGTC AATGACCTAA TCCTGGAGGG CCCAGCCTTG CCTATCATGC 1621 ACATGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1681 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1741 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAAATGAAGA TTTCGAAACT TTGAAACGCG 1801 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt