Construct: ORF TRCN0000470307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000598.1_s317c1
- Derived from:
- ccsbBroadEn_13423
- DNA Barcode:
- TCCCTAACAATTGTCGTAGCGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NT5DC1 (221294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221294 | NT5DC1 | 5'-nucleotidase domain cont... | NM_152729.3 | 62.4% | 62.4% | 1_513del |
| 2 | human | 221294 | NT5DC1 | 5'-nucleotidase domain cont... | XM_006715378.3 | 30.7% | 30.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agcttttaag gaaaactgtg gaatatattt tccagaaata aaaagagatc 121 caggcagata tttacatagt tgtcctgaat ctgtgaaaaa atggcttcga cagctaaaga 181 atgctgggaa aattcttctg ttaattacca gttctcacag tgattactgt agacttctct 241 gcgaatatat tcttgggaat gattttacag acctttttga cattgtgatt acaaatgcat 301 tgaagcctgg tttcttctcc cacttaccaa gtcagagacc tttccggaca ctcgagaatg 361 atgaggagca ggaggcactg ccatctctgg ataaacctgg ctggtactcc caagggaacg 421 ctgtccacct ctatgaactt ctgaagaaaa tgactggcaa accTGAACCC AAGGTTGTTT 481 ATTTTGGTGA CAGCATGCAT TCAGATATTT TCCCAGCTCG TCACTATAGT AATTGGGAGA 541 CAGTCCTCAT CCTGGAAGAA CTCAGAGGGG ATGAAGGCAC GAGGAGTCAG AGGCCTGAGG 601 AGTCAGAGCC TCTAGAGAAG AAAGGAAAAT ATGAGGGACC AAAAGCAAAA CCTTTAAATA 661 CTTCATCTAA AAAATGGGGC TCTTTTTTTA TTGATTCAGT TTTGGGACTG GAAAATACAG 721 AAGACTCCTT GGTTTATACA TGGTCTTGTA AGAGAATCAG TACTTACAGC ACTATTGCAA 781 TTCCAAGTAT TGAAGCAATC GCAGAATTAC CTCTGGACTA CAAATTTACA AGATTCTCTT 841 CAAGCAATTC AAAAACAGCT GGCTACTATC CAAATCCTCC ACTGGTCTTA TCAAGTGATG 901 AGACACTGAT ATCCAAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCCCTAA CAATTGTCGT 1081 AGCGTCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt