Construct: ORF TRCN0000470318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002357.1_s317c1
- Derived from:
- ccsbBroadEn_10932
- DNA Barcode:
- AGATCCCTGCCGCGAGGGTCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNE1 (3753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_000219.6 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 2 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001127668.3 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 3 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001127669.4 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 4 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001127670.3 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 5 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001270402.2 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 6 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001270403.2 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 7 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001270404.2 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 8 | human | 3753 | KCNE1 | potassium voltage-gated cha... | NM_001270405.2 | 80.8% | 79.8% | 95G>A;112A>G;316_387del |
| 9 | human | 102723475 | KCNE1B | potassium voltage-gated cha... | NM_001330065.1 | 79% | 78% | (many diffs) |
| 10 | human | 102723475 | KCNE1B | potassium voltage-gated cha... | NM_001369869.1 | 79% | 78% | (many diffs) |
| 11 | human | 102723475 | KCNE1B | potassium voltage-gated cha... | XM_006723946.2 | 69.5% | 68.6% | (many diffs) |
| 12 | human | 3753 | KCNE1 | potassium voltage-gated cha... | XM_017028342.1 | 69.5% | 68.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAT CCTGTCTAAC ACCACAGCGG TGACGCCCTT TCTGACCAAG CTGTGGCAGG 121 AGACAGTTCA GCAGGGTGGC AACATGTCGG GCCTGGCCCA CAGGTCCCCC CGCAGCGGTG 181 ACGGCAAGCT GGAGGCCCTC TACGTCCTCA TGGTACTGGG ATTCTTCGGC TTCTTCACCC 241 TGGGCATCAT GCTGAGCTAC ATCCGCTCCA AGAAGCTGGA GCACTCGAAC GACCCATTCA 301 ACGTCTACAT CGAGTCCGAT GCCTGGCAAG AGAAGGACAA GGCCTATGTC CAGGCCCGGG 361 TCCTGGAGAG CTACAGGTCG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGAT CCCTGCCGCG 541 AGGGTCTTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt