Construct: ORF TRCN0000470319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015544.1_s317c1
- Derived from:
- ccsbBroadEn_08280
- DNA Barcode:
- TCTCGGTCTATATCAGCGATGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POP5 (51367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51367 | POP5 | POP5 homolog, ribonuclease ... | NM_015918.4 | 99.7% | 100% | 442T>C |
| 2 | human | 51367 | POP5 | POP5 homolog, ribonuclease ... | XM_011538441.1 | 89.8% | 90% | 312_365del;496T>C |
| 3 | human | 51367 | POP5 | POP5 homolog, ribonuclease ... | NM_198202.1 | 69.1% | 69.3% | 162_163ins150;292T>C |
| 4 | mouse | 117109 | Pop5 | processing of precursor 5, ... | NM_026398.4 | 82.6% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcggttcaag cacaggtacc tgctctgcga actggtgtct gacgaccccc 121 gctgccgcct aagcctcgat gaccgagttc tgagcagcct cgtacgggac acgatcgcca 181 gggtgcacgg aactttcggc gcagccgccT GCTCCATCGG CTTCGCGGTT CGATATCTCA 241 ATGCCTATAC TGGAATAGTG CTACTTCGAT GCAGAAAAGA ATTCTATCAG CTTGTGTGGT 301 CAGCTCTTCC CTTCATCACA TACTTGGAGA ACAAAGGACA CCGTTACCCA TGCTTTTTCA 361 ACACATTACA TGTGGGAGGT ACAATAAGAA CATGTCAGAA GTTCCTAATT CAGTACAACA 421 GGAGACAGCT GTTGATCTTG TTGCAGAACT GCACTGATGA AGGAGAGCGG GAAGCTATCC 481 AGAAGTCTGT GACAAGAAGC TGCTTACTAG AGGAGGAGGA GGAGTCAGGT GAGGAGGCTG 541 CAGAAGCAAT GGAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCTCGGTCTA TATCAGCGAT 721 GACGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt