Construct: ORF TRCN0000470327

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009098.1_s317c1
Derived from:
ccsbBroadEn_06093
DNA Barcode:
TGCGATAGTATCGTCGGGATTCTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DES (1674)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470327
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1674 DES desmin NM_001927.4 99.5% 100% (many diffs)
2 mouse 13346 Des desmin NM_010043.2 90.9% 97.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1476
ORF length:
1410
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag ccaggcctac tcgtccagcc agcgcgtgtc ctcctaccgc cgcaccttcg
121gcggggcccc gggcttcccg ctcggctccc cgctgagctc gcccgtgttc ccgcgggcgg
181gtttcggctc taagggctcc tccagctcgg tgacgtcccg cgtgtaccag gtgtcgcgca
241cgtcgggcgg ggccgggggc ctggggtcgc tgcgggccag ccggctgggg accacccgca
301cgccctcctc ctacggcgca ggcgagctgc tggacttctc actggccgac gcggtgaacc
361aggagtttct gaccacgcgc accaacgaga aggtggagct gcaggagctc aatgaccgct
421tcgccaacta catcgagaag gtgcgcttcc tggagcagca gaacgcggcg ctcgccgccg
481aagtgaaccg gctcaagggc cgcgagccga cgcgagtggc cgagctctac gaggaggagc
541tgcgggagct gcggcgccag gtggaggtgc tcactaacca gcgcgcgcgc gtcgacgtcg
601agcgcgacaa cctgctcgac gacctgcagc ggctcaaggc caagctgcag gaggagattc
661agttgaagga agaagcagag aacaatttgg ctgccttccg agcggacgtg gatgcagcta
721ctctagctcg cattgacctg gagcgcagaa ttgaatctct caacgaggag atcgcgttcc
781ttaagaaagt gcatgaagag gagatccgtg agttgcaggc tcagcttcag gaacagcagg
841tccaggtgga gatggacatg tctaagccag acctcactgc cgccctcagg gatatccggg
901ctcagtatga gaccatcgcg gctaagaaca tttctgaagc tgaggagtgg tacaagtcga
961aggtgtcaga cctgacccag gcagccaaca agaacaacga cgccctgcgc caggccaagc
1021aggagatgat ggaataccga caccagatcc agtcctacac ctgcgagatt gacgccctca
1081agggcactaa cgattccctg atgaggcaga tgcgggaatt ggaggaccga tttGCCAGTG
1141AGGCCAGTGG CTACCAGGAC AACATTGCAC GCCTGGAGGA GGAAATCCGG CACCTCAAGG
1201ATGAGATGGC CCGCCATCTG CGCGAGTACC AGGACCTGCT CAACGTGAAG ATGGCCCTGG
1261ATGTGGAGAT TGCCACCTAC CGGAAGCTGC TGGAGGGAGA GGAGAGCCGG ATCAATCTCC
1321CCATCCAGAC CTACTCTGCC CTCAATTTCC GAGAAACCAG CCCTGAGCAA AGGGGTTCTG
1381AGGTCCATAC CAAGAAGACG GTGATGATCA AGACCATCGA GACACGGGAT GGGGAGGTCG
1441TCAGTGAGGC CACACAGCAG CAGCATGAAG TGCTCTACCC AACTTTCTTG TACAAAGTGG
1501TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1561CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1621ATGCGATAGT ATCGTCGGGA TTCTGACGCG TTAAGTCgac aatcaacctc tggattacaa
1681aatttgtgaa agatt

Download FASTA (ORF) (Full)