Construct: ORF TRCN0000470327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009098.1_s317c1
- Derived from:
- ccsbBroadEn_06093
- DNA Barcode:
- TGCGATAGTATCGTCGGGATTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DES (1674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1674 | DES | desmin | NM_001927.4 | 99.5% | 100% | (many diffs) |
| 2 | mouse | 13346 | Des | desmin | NM_010043.2 | 90.9% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1476
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccaggcctac tcgtccagcc agcgcgtgtc ctcctaccgc cgcaccttcg 121 gcggggcccc gggcttcccg ctcggctccc cgctgagctc gcccgtgttc ccgcgggcgg 181 gtttcggctc taagggctcc tccagctcgg tgacgtcccg cgtgtaccag gtgtcgcgca 241 cgtcgggcgg ggccgggggc ctggggtcgc tgcgggccag ccggctgggg accacccgca 301 cgccctcctc ctacggcgca ggcgagctgc tggacttctc actggccgac gcggtgaacc 361 aggagtttct gaccacgcgc accaacgaga aggtggagct gcaggagctc aatgaccgct 421 tcgccaacta catcgagaag gtgcgcttcc tggagcagca gaacgcggcg ctcgccgccg 481 aagtgaaccg gctcaagggc cgcgagccga cgcgagtggc cgagctctac gaggaggagc 541 tgcgggagct gcggcgccag gtggaggtgc tcactaacca gcgcgcgcgc gtcgacgtcg 601 agcgcgacaa cctgctcgac gacctgcagc ggctcaaggc caagctgcag gaggagattc 661 agttgaagga agaagcagag aacaatttgg ctgccttccg agcggacgtg gatgcagcta 721 ctctagctcg cattgacctg gagcgcagaa ttgaatctct caacgaggag atcgcgttcc 781 ttaagaaagt gcatgaagag gagatccgtg agttgcaggc tcagcttcag gaacagcagg 841 tccaggtgga gatggacatg tctaagccag acctcactgc cgccctcagg gatatccggg 901 ctcagtatga gaccatcgcg gctaagaaca tttctgaagc tgaggagtgg tacaagtcga 961 aggtgtcaga cctgacccag gcagccaaca agaacaacga cgccctgcgc caggccaagc 1021 aggagatgat ggaataccga caccagatcc agtcctacac ctgcgagatt gacgccctca 1081 agggcactaa cgattccctg atgaggcaga tgcgggaatt ggaggaccga tttGCCAGTG 1141 AGGCCAGTGG CTACCAGGAC AACATTGCAC GCCTGGAGGA GGAAATCCGG CACCTCAAGG 1201 ATGAGATGGC CCGCCATCTG CGCGAGTACC AGGACCTGCT CAACGTGAAG ATGGCCCTGG 1261 ATGTGGAGAT TGCCACCTAC CGGAAGCTGC TGGAGGGAGA GGAGAGCCGG ATCAATCTCC 1321 CCATCCAGAC CTACTCTGCC CTCAATTTCC GAGAAACCAG CCCTGAGCAA AGGGGTTCTG 1381 AGGTCCATAC CAAGAAGACG GTGATGATCA AGACCATCGA GACACGGGAT GGGGAGGTCG 1441 TCAGTGAGGC CACACAGCAG CAGCATGAAG TGCTCTACCC AACTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 ATGCGATAGT ATCGTCGGGA TTCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt