Construct: ORF TRCN0000470329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000127.1_s317c1
- Derived from:
- ccsbBroadEn_12225
- DNA Barcode:
- GCACCGATACCGATCCCCCCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT10 (55568)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55568 | GALNT10 | polypeptide N-acetylgalacto... | NM_198321.4 | 32.8% | 32% | (many diffs) |
| 2 | mouse | 171212 | Galnt10 | UDP-N-acetyl-alpha-D-galact... | NM_134189.2 | 29.5% | 29.8% | (many diffs) |
| 3 | mouse | 171212 | Galnt10 | UDP-N-acetyl-alpha-D-galact... | XM_006532389.3 | 15.2% | 15.7% | (many diffs) |
| 4 | mouse | 171212 | Galnt10 | UDP-N-acetyl-alpha-D-galact... | XM_006532390.3 | 15.2% | 15.7% | (many diffs) |
| 5 | mouse | 171212 | Galnt10 | UDP-N-acetyl-alpha-D-galact... | XM_006532391.2 | 15.2% | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 672
- ORF length:
- 606
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcggaaggag aagcggctcc tgcaggcggt ggcgctggtg ctggcggccc 121 tggtcctcct gcccaacgtg gggctttggg cgctgtaccg cgagcggcag cccgacggca 181 cccctggggg atcgggggcg gcggtggcgc cggcggcggg acagggctca cacagtcgac 241 aaaagaaaac gtttttcttg ggagatgggc agaagctgaa ggactggcat gacaaggagg 301 CCATCCGGAG GGACGCTCAG CGCGTAGGAA ATGGAGAACA AGGAAGACCT TACCCCATGA 361 CCGATGCTGA GAGAGTGGAT CAGGCATACC GAGAAAATGG ATTTAACATC TACGTCAGTG 421 ATAAAATCTC CTTGAATCGC TCTCTCCCAG ATATCCGGCA CCCAAACTGC AACAGCAAGC 481 GCTACCTGGA GACACTTCCC AACACAAGCA TCATCATCCC CTTCCACAAC GAGGGCTGGT 541 CCTCCCTCCT CCGCACCGTC CACAGTGTGC TCAATCGCTC GCCTCCAGAG CTGGTCGCCG 601 AGATTGTACT GGTCGACGAC TTCAGTGATC GAGATCTGCC GACTGCATCA ACTCCCTCTC 661 CCTTTGTGTG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCA CCGATACCGA TCCCCCCAAT 841 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t