Construct: ORF TRCN0000470333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006666.1_s317c1
- Derived from:
- ccsbBroadEn_04461
- DNA Barcode:
- GAGGACCCAGAGGCGATACGGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM83A (84985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84985 | FAM83A | family with sequence simila... | NM_032899.6 | 100% | 100% | |
| 2 | human | 84985 | FAM83A | family with sequence simila... | XM_005251087.3 | 87% | 87% | 478_479ins168 |
| 3 | human | 84985 | FAM83A | family with sequence simila... | NM_207006.2 | 78% | 77% | (many diffs) |
| 4 | human | 84985 | FAM83A | family with sequence simila... | NM_001288587.3 | 67.5% | 67% | (many diffs) |
| 5 | human | 84985 | FAM83A | family with sequence simila... | NM_001321630.2 | 61.3% | 60% | (many diffs) |
| 6 | human | 84985 | FAM83A | family with sequence simila... | XM_011517338.2 | 59.6% | 59.4% | (many diffs) |
| 7 | mouse | 239463 | Fam83a | family with sequence simila... | XM_017316599.1 | 47.6% | 44.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccggtcaagg cacctgggca aaatccggaa gcgtctggaa gatgtcaaga 121 gccagtgggt ccggccagcc agggctgact ttagtgacaa cgagagtgcc cggctggcca 181 cggacgccct cttggatggg ggttctgaag cctactggcg ggtgctcagc caggaaggcg 241 aggtggactt cttgtcctcg gtggaggccc agtacatcca ggcccaggcc agggagcccc 301 cgtgtccccc agacaccctg ggaggggcgg aagcaggccc taagggactg gactccagct 361 ccctacagtc cggcacctac ttccctgtgg cctcagaggg cagcgagccg gccctactgc 421 acagctgggc ctcagctgag aagccctacc tgaaggaaaa atccagcgcc actgtgtact 481 tccagaccgt caagcacaac aacatcagag acctcgtccg ccgctgcatc acccggacta 541 gccaggtcct ggtcatcctg atggatgtgt tcacggatgt ggagatcttc tgtgacattc 601 tagaggcagc caacaagcgt ggggtgttcg tttgtgtgct cctggaccag ggaggtgtga 661 agctcttcca ggagatgtgt gacaaagtcc agatctctga cagtcacctc aagaacattt 721 ccatccggag tgtggaagga gagatatact gtgccaagtc aggcaggaaa ttcgctggcc 781 aaatccggga gaagttcatc atctcggact ggagatttgt cctgtctgga tcttacagct 841 tcacctggct ctgcggacac gtgcaccgga acatcctctc caagttcaca ggccaggcgg 901 tggagctgtt tgacgaggag ttccgccacc tctacgcctc ctccaagcct gtgatgggcc 961 tgaagtcccc gcggctggtc gcccccgtcc cgcccggagc agccccggCC AATGGCCGCC 1021 TTAGCAGCAG CAGTGGCTCC GCCAGTGACC GCACGTCCTC CAACCCCTTC AGCGGCCGCT 1081 CGGCAGGCAG CCACCCCGGT ACCCGAAGTG TGTCCGCGTC TTCAGGGCCC TGTAGCCCCG 1141 CGGCCCCACA CCCGCCTCCA CCGCCCCGGT TCCAGCCCCA CCAAGGCCCT TGGGGAGCCC 1201 CGAGTCCCCA GGCCCACCTC TCCCCGCGGC CCCACGACGG CCCGCCCGCC GCTGTCTACA 1261 GCAACCTGGG GGCCTACAGG CCCACGCGGC TGCAGCTGGA GCAGCTGGGC CTGGTGCCGA 1321 GGCTGACTCC AACCTGGAGG CCCTTCCTGC AGGCCTCCCC TCACTTCTGC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAGAGGACC CAGAGGCGAT ACGGGTGACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt