Construct: ORF TRCN0000470334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009419.1_s317c1
- Derived from:
- ccsbBroadEn_05989
- DNA Barcode:
- TGTTCAACCACATTCTAATTAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1C4 (1109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470334
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1109 | AKR1C4 | aldo-keto reductase family ... | NM_001818.4 | 99.8% | 99.6% | 509G>A |
| 2 | human | 1645 | AKR1C1 | aldo-keto reductase family ... | NM_001353.6 | 88.9% | 82.9% | (many diffs) |
| 3 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001354.5 | 88.4% | 81.7% | (many diffs) |
| 4 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_205845.2 | 88.4% | 81.7% | (many diffs) |
| 5 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_003739.6 | 88.4% | 84.2% | (many diffs) |
| 6 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_001253908.1 | 88.1% | 83.9% | (many diffs) |
| 7 | human | 1645 | AKR1C1 | aldo-keto reductase family ... | XM_017015791.2 | 82.1% | 76.7% | (many diffs) |
| 8 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001321027.1 | 81.6% | 75.5% | (many diffs) |
| 9 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_001253909.2 | 37.9% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcccaaatat cagcgtgtag agctaaatga tggtcacttc atgcccgtat 121 tgggatttgg cacctatgca cctccagagg ttccgaggaa cagagctgta gaggtcacca 181 aattagcaat agaagctggc ttccgccata ttgattctgc ttatttatac aataatgagg 241 agcaggttgg actggccatc cgaagcaaga ttgcagatgg cagtgtgaag agagaagaca 301 tattctacac ttcaaagctt tggtgcactt tctttcaacc acagatggtc caaccagcct 361 tggaaagctc actgaaaaaa cttcaactgg actatgttga cctctatctt cttcatttcc 421 caatggctct caagccaggt gagacgccac taccaaaaga tgaaaatgga aaagtaatat 481 tcgacacagt ggatctctct gccacatggg aggtcatgga gaagtgtaag gatgcaggat 541 tggccaagtc catcggggtg tcaaacttca actacaggca gctggagatg atcctcaaca 601 agccaggact caagtacaag cctgtctgca accaggtaga atgtcatcct taccTCAACC 661 AGAGCAAACT GCTGGATTTC TGCAAGTCAA AAGACATTGT TCTGGTTGCC CACAGTGCTC 721 TGGGAACCCA ACGACATAAA CTATGGGTGG ACCCAAACTC CCCAGTTCTT TTGGAGGACC 781 CAGTTCTTTG TGCCTTAGCA AAGAAACACA AACGAACCCC AGCCCTGATT GCCCTGCGCT 841 ACCAGCTGCA GCGTGGGGTT GTGGTCCTGG CCAAGAGCTA CAATGAGCAG CGGATCAGAG 901 AGAACATCCA GGTTTTTGAA TTCCAGTTGA CATCAGAGGA TATGAAAGTT CTAGATGGTC 961 TAAACAGAAA TTATCGATAT GTTGTCATGG ATTTTCTTAT GGACCATCCT GATTATCCAT 1021 TTTCAGATGA ATATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGTTCAACCA CATTCTAATT 1201 AGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt