Construct: ORF TRCN0000470339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016278.1_s317c1
- Derived from:
- ccsbBroadEn_06008
- DNA Barcode:
- TCACACTTGAAGGGCTCTATCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP2M1 (1173)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1173 | AP2M1 | adaptor related protein com... | NM_001025205.1 | 99.7% | 99.7% | 13T>G;954C>T;1179G>A |
| 2 | human | 1173 | AP2M1 | adaptor related protein com... | NM_004068.4 | 99.3% | 99.3% | (many diffs) |
| 3 | human | 1173 | AP2M1 | adaptor related protein com... | NM_001311198.1 | 93.9% | 93.9% | (many diffs) |
| 4 | mouse | 11773 | Ap2m1 | adaptor-related protein com... | NM_001302970.1 | 92.5% | 99.7% | (many diffs) |
| 5 | mouse | 11773 | Ap2m1 | adaptor-related protein com... | NM_009679.3 | 92.1% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tggaggcgta ttcatctata atcacaaggg ggaggtgctc atctcccgag 121 tctaccgaga tgacatcggg aggaacgcag tggatgcctt tcgggtcaat gttatccatg 181 cccggcagca ggtgcgcagc cccgtcacca acattgctcg caccagcttc ttccacgtta 241 agcggtccaa catttggctg gcagcagtca ccaagcagaa tgtcaacgct gccatggtct 301 tcgaattcct ctataagatg tgtgacgtga tggctgccta ctttggcaag atcagcgagg 361 aaaacatcaa gaacaatttt gtgctcatat atgagctgct ggatgagatt ctagactttg 421 gctacccaca gaattccgag acaggcgcgc tgaaaacctt catcacgcag cagggcatca 481 agagtcagac aaaagaagag cagtcacaga tcaccagcca ggtaactggg cagattggct 541 ggcggcgaga gggtatcaag tatcgtcgga atgagctctt cctggatgtg ctggagagtg 601 tgaacctgct catgtcccca caagggcagg tgctgagtgc ccatgtgtcg ggccgggtgg 661 tgatgaagag ctacctgagt ggcatgcctg aatgcaagtt tgggatgaat gacaagattg 721 ttattgaaaa gcagggcaaa ggcacagctg atgaaacaag caagagcggg aagcaatcaa 781 ttgccattga tgactgcacc ttccaccagt gtgtgcgact cagcaagttt gactctgaac 841 gcagcatcag ctttatcccg ccagatggag agtttgagct tatgaggtat cgcacaacca 901 aggacatcat ccTTCCCTTC CGGGTGATCC CGCTAGTGCG AGAAGTGGGA CGCACCAAAC 961 TGGAGGTCAA GGTGGTCATC AAGTCCAACT TTAAACCCTC ACTGCTGGCT CAGAAGATTG 1021 AGGTGAGGAT CCCAACCCCA CTGAACACAA GCGGGGTGCA GGTGATCTGC ATGAAGGGGA 1081 AGGCCAAGTA CAAGGCCAGC GAGAATGCCA TCGTGTGGAA GATCAAGCGC ATGGCAGGCA 1141 TGAAGGAATC GCAGATCAGC GCAGAGATTG AGCTTCTGCC TACCAACGAC AAGAAGAAAT 1201 GGGCTCGACC CCCCATTTCC ATGAACTTTG AGGTGCCATT CGCACCCTCT GGCCTCAAGG 1261 TGCGCTACTT GAAGGTGTTT GAACCGAAGC TGAACTACAG CGACCATGAT GTCATCAAAT 1321 GGGTGCGCTA CATTGGCCGC AGTGGCATTT ATGAAACTCG CTGCTACCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA TCACACTTGA AGGGCTCTAT CACCACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt