Construct: ORF TRCN0000470349
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005383.1_s317c1
- Derived from:
- ccsbBroadEn_06438
- DNA Barcode:
- AATTTAGACTTGGATATCTTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL2RB (3560)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470349
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3560 | IL2RB | interleukin 2 receptor subu... | NM_000878.5 | 99.9% | 100% | 750C>T |
| 2 | human | 3560 | IL2RB | interleukin 2 receptor subu... | NM_001346222.1 | 99.9% | 100% | 750C>T |
| 3 | human | 3560 | IL2RB | interleukin 2 receptor subu... | NM_001346223.2 | 99.9% | 100% | 750C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1719
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcccctgct ctgtcctggc gtctgcccct cctcatcctc ctcctgcccc 121 tggctacctc ttgggcatct gcagcggtga atggcacttc ccagttcaca tgcttctaca 181 actcgagagc caacatctcc tgtgtctgga gccaagatgg ggctctgcag gacacttcct 241 gccaagtcca tgcctggccg gacagacggc ggtggaacca aacctgtgag ctgctccccg 301 tgagtcaagc atcctgggcc tgcaacctga tcctcggagc cccagattct cagaaactga 361 ccacagttga catcgtcacc ctgagggtgc tgtgccgtga gggggtgcga tggagggtga 421 tggccatcca ggacttcaag ccctttgaga accttcgcct gatggccccc atctccctcc 481 aagttgtcca cgtggagacc cacagatgca acataagctg ggaaatctcc caagcctccc 541 actactttga aagacacctg gagttcgagg cccggacgct gtccccaggc cacacctggg 601 aggaggcccc cctgctgact ctcaagcaga agcaggaatg gatctgcctg gagacgctca 661 ccccagacac ccagtatgag tttcaggtgc gggtcaagcc tctgcaaggc gagttcacga 721 cctggagccc ctggagccag cccctggcct tcaggacaaa gcctgcagcc cttgggaagg 781 acaccattcc gtggctcggc cacctcctcg tgggtctcag cggggctttt ggcttcatca 841 tcttagtgta cttgctgatc aactgcagga acaccgggcc atggctgaag aaggtcctga 901 agtgtaacac cccagacccc tcgaagttct tttcccagct gagctcagag catggaggag 961 acgtccagaa gtggctctct tcgcccttcc cctcatcgtc cttcagccct ggcggcctgg 1021 cacctgagat ctcgccacta gaagtgctgg agagggacaa ggtgacgcag ctgctcctgc 1081 agcaggacaa ggtgcctgag cccgcatcct taagcagcaa ccactcgctg accagctgct 1141 tcaccaacca gggttacttc ttcttccacc tcccggatgc cttggagata gaggcctgcc 1201 aggtgtactt tacttacgac ccctactcag aggaagaccc tgatgagggt gtggccgggg 1261 cacccacagg gtcttccccc caacccctgc agccTCTGTC AGGGGAGGAC GACGCCTACT 1321 GCACCTTCCC CTCCAGGGAT GACCTGCTGC TCTTCTCCCC CAGTCTCCTC GGTGGCCCCA 1381 GCCCCCCAAG CACTGCCCCT GGGGGCAGTG GGGCCGGTGA AGAGAGGATG CCCCCTTCTT 1441 TGCAAGAAAG AGTCCCCAGA GACTGGGACC CCCAGCCCCT GGGGCCTCCC ACCCCAGGAG 1501 TCCCAGACCT GGTGGATTTT CAGCCACCCC CTGAGCTGGT GCTGCGAGAG GCTGGGGAGG 1561 AGGTCCCTGA CGCTGGCCCC AGGGAGGGAG TCAGTTTCCC CTGGTCCAGG CCTCCTGGGC 1621 AGGGGGAGTT CAGGGCCCTT AATGCTCGCC TGCCCCTGAA CACTGATGCC TACTTGTCCC 1681 TCCAAGAACT CCAGGGTCAG GACCCAACTC ACTTGGTGTA CCCAACTTTC TTGTACAAAG 1741 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1801 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1861 ACGAAATTTA GACTTGGATA TCTTCCGGAC GCGTTAAGTC gacaatcaac ctctggatta 1921 caaaatttgt gaaagatt