Construct: ORF TRCN0000470355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002914.1_s317c1
- Derived from:
- ccsbBroadEn_01673
- DNA Barcode:
- AAGTCCCTTATCAGGAATCAGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMP3 (7078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7078 | TIMP3 | TIMP metallopeptidase inhib... | NM_000362.4 | 100% | 100% | |
| 2 | mouse | 21859 | Timp3 | tissue inhibitor of metallo... | NM_011595.2 | 89.7% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cccttggctc gggctcatcg tgctcctggg cagctggagc ctgggggact 121 ggggcgccga ggcgtgcaca tgctcgccca gccaccccca ggacgccttc tgcaactccg 181 acatcgtgat ccgggccaag gtggtgggga agaagctggt aaaggagggg cccttcggca 241 cgctggtcta caccatcaag cagatgaaga tgtaccgagg cttcaccaag atgccccatg 301 tgcagtacat ccatacggaa gcttccgaga gtctctgtgg ccttaagctg gaggtcaaca 361 agtaccagta cctgctgaca ggtcgcgtcT ATGATGGCAA GATGTACACG GGGCTGTGCA 421 ACTTCGTGGA GAGGTGGGAC CAGCTCACCC TCTCCCAGCG CAAGGGGCTG AACTATCGGT 481 ATCACCTGGG TTGTAACTGC AAGATCAAGT CCTGCTACTA CCTGCCTTGC TTTGTGACTT 541 CCAAGAACGA GTGTCTCTGG ACCGACATGC TCTCCAATTT CGGTTACCCT GGCTACCAGT 601 CCAAACACTA CGCCTGCATC CGGCAGAAGG GCGGCTACTG CAGCTGGTAC CGAGGATGGG 661 CCCCCCCGGA TAAAAGCATC ATCAATGCCA CAGACCCCTG CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGAAAGTCC CTTATCAGGA ATCAGAAGAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt