Construct: ORF TRCN0000470359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014976.1_s317c1
- Derived from:
- ccsbBroadEn_04897
- DNA Barcode:
- TGAGTCATAATAGCTTTCTTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLVS2 (134829)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134829 | CLVS2 | clavesin 2 | NM_001010852.4 | 100% | 100% | |
| 2 | mouse | 215890 | Clvs2 | clavesin 2 | NM_175448.3 | 90.7% | 97.8% | (many diffs) |
| 3 | mouse | 215890 | Clvs2 | clavesin 2 | XM_017313882.1 | 90.7% | 97.8% | (many diffs) |
| 4 | mouse | 215890 | Clvs2 | clavesin 2 | XM_006512693.2 | 49.5% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1050
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactcatttg caagccggtc tctcccctga gaccctggag aaagctcgcc 121 tggagctcaa tgaaaaccca gacacgctgc accaggacat ccaggaggtg agggatatgg 181 tcatcaccag gccggacatt ggctttctgc gcacggatga tgccttcatc ttacgcttct 241 tgcgggctag gaagtttcat cactttgagg ccttccgcct cctggcgcag tactttgagt 301 accggcagca gaacctggac atgttcaaaa gctttaaggc caccgaccct ggcatcaagc 361 aggcactgaa ggatggcttc cctgggggcc tggccaatct ggaccactat ggcaggaaga 421 ttctagtcct ttttgctgcc aattgggatc agagcaggta cacactggtg gatattttgc 481 gtgccatctt actttcttta gaagccatga ttgaagatcc tgagcttcaa gtgaatgggt 541 ttgttttgat catagactgg agtaacttca ctttcaagca agcctctaaa ctcacaccaa 601 gtatgctgcg attagctatt gaaggcctgc aggatagttt cccagcgcga tttggaggaa 661 ttcattttgt caatcaacca tggtatatcc atgccctgta caccgtgatc cggcctttcc 721 tgaaggagaa aactcggaaa aggatatttt tgcatggtaa caacctgaac agtctacacc 781 aactaattca tcctgagatc ctgccctctg agtttggagg aatgctgcct ccttatgaca 841 tggggacatg ggcaagaaca ctgctagacc atgaatatga cgatgacagc gagtacaatg 901 tagactccTA CAGCATGCCT GTGAAGGAAG TAGAGAAGGA ACTCTCCCCA AAGTCCATGA 961 AGAGATCTCA ATCAGTAGTG GATCCTACAG TACTAAAACG CATGGATAAA AATGAGGAAG 1021 AAAACATGCA ACCATTGCTT TCTCTGGACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGAGT 1201 CATAATAGCT TTCTTATCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt