Construct: ORF TRCN0000470366
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013790.1_s317c1
- Derived from:
- ccsbBroadEn_12423
- DNA Barcode:
- TTCTATTCTGAGCAGATGTTTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCPEP1 (59342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470366
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59342 | SCPEP1 | serine carboxypeptidase 1 | XM_005257557.3 | 71% | 70.7% | 881_897del;901_902insGTT;903_1242del |
| 2 | human | 59342 | SCPEP1 | serine carboxypeptidase 1 | NM_021626.3 | 65.3% | 65% | 885_886delTCinsGT;889_1356del |
| 3 | human | 59342 | SCPEP1 | serine carboxypeptidase 1 | XR_001752578.2 | 37.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctggcactg cggcgctctc ccgtcccgcg gtggttgctg ctgctgccgc 121 tgctgctggg cctgaacgca ggagctgtca ttgactggcc cacagaggag ggcaaggaag 181 tatgggatta tgtgacggtc cgcaaggatg cctacatgtt ctggtggctc tattatgcca 241 ccaactcctg caagaacttc tcagaactgc ccctggtcat gtggcttcag ggcggtccag 301 gcggttctag cactggattt ggaaactttg aggaaattgg gccccttgac agtgatctca 361 aaccacggaa aaccacctgg ctccaggctg ccagtctcct atttgtggat aatcccgtgg 421 gcactgggtt cagttatgtg aatggtagtg gtgcctatgc caaggacctg gctatggtgg 481 cttcagacat gatggttctc ctgaagaccT TCTTCAGTTG CCACAAAGAA TTCCAGACAG 541 TTCCATTCTA CATTTTCTCA GAGTCCTATG GAGGAAAAAT GGCAGCTGGC ATTGGTCTAG 601 AGCTTTATAA GGCCATTCAG CGAGGGACCA TCAAGTGCAA CTTTGCGGGG GTTGCCTTGG 661 GTGATTCCTG GATCTCCCCT GTTGATTCGG TGCTCTCCTG GGGACCTTAC CTGTACAGCA 721 TGTCTCTTCT CGAAGACAAA GGTCTGGCAG AGGTGTCTAA GGTTGCAGAG CAAGTACTGA 781 ATGCCGTAAA TAAGGGGCTC TACAGAGAGG CCACAGAGCT GTGGGGGAAA GCAGAAATGA 841 TCATTGAACA GAACACAGAT GGGGTGAACT TCTATAACAT CTTAACTAAA AGCACTCCCA 901 CGTCTACAAT GGAGTCGAGT CTAGAATTCA CACAGAGCCA CCTAGTTTGG TTTTACCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAT TCTATTCTGA GCAGATGTTT CCCACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att