Construct: ORF TRCN0000470384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012573.1_s317c1
- Derived from:
- ccsbBroadEn_07272
- DNA Barcode:
- TCATTTAGAAATTCTAAGTCCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLPP3 (8613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8613 | PLPP3 | phospholipid phosphatase 3 | NM_003713.5 | 99.8% | 99.6% | 845C>T |
| 2 | mouse | 67916 | Plpp3 | phospholipid phosphatase 3 | NM_080555.2 | 88.7% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca aaactacaag tacgacaaag cgatcgtccc ggagagcaag aacggcggca 121 gcccggcgct caacaacaac ccgaggagga gcggcagcaa gcgggtgctg ctcatctgcc 181 tcgacctctt ctgcctcttc atggcgggcc tccccttcct catcatcgag acaagcacca 241 tcaagcctta ccaccgaggg ttttactgca atgatgagag catcaagtac ccactgaaaa 301 ctggtgagac aataaatgac gctgtgctct gtgccgtggg gatcgtcatt gccatcctcg 361 cgatcatcac gggggaattc taccggatct attacctgaa gaagtcgcgg tcgacgattc 421 agaaccccta cgtggcagca ctctataagc aagtgggctg cttcctcttt ggctgtgcca 481 tcagccagtc tttcacagac attgccaaag tgtccatagg gcgcctgcgt cctcacttct 541 tgagtgtctg caaccctgat ttcagccaga tcaactgctc tgaaggctac attcagaact 601 acagatgcag aggtgatgac agcaaagtcc aggaagccag gaagtccttc ttctctggcc 661 atgcctcctt ctccatgtac actatgctgt atttgGTGCT ATACCTGCAG GCCCGCTTCA 721 CTTGGCGAGG AGCCCGCCTG CTCCGGCCCC TCCTGCAGTT CACCTTGATC ATGATGGCCT 781 TCTACACGGG ACTGTCTCGC GTATCAGACC ACAAGCACCA TCCCAGTGAT GTTCTGGCAG 841 GATTTGCTCA AGGAGCCCTG GTGGCCTGCT GCATAGTTTT CTTCGTGTCT GACCTCTTCA 901 AGACTAAGAT GACGCTCTCC CTGCCTGCCC CTGCTATCCG GAAGGAAATC CTTTCACCTG 961 TGGACATTAT TGACAGGAAC AATCACCACA ACATGATGTA CCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGATCATTT AGAAATTCTA AGTCCGGTAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt