Construct: ORF TRCN0000470386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008462.1_s317c1
- Derived from:
- ccsbBroadEn_12537
- DNA Barcode:
- AAGAGCTGTGTTTCACCTTGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPM8 (79054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79054 | TRPM8 | transient receptor potentia... | NM_024080.5 | 16.3% | 15.3% | (many diffs) |
| 2 | human | 79054 | TRPM8 | transient receptor potentia... | XM_011511810.2 | 16.1% | 15.2% | (many diffs) |
| 3 | human | 79054 | TRPM8 | transient receptor potentia... | XM_024453132.1 | 15.2% | 14.8% | (many diffs) |
| 4 | human | 79054 | TRPM8 | transient receptor potentia... | XM_017004891.1 | 15.1% | 14.6% | (many diffs) |
| 5 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_017319118.1 | 21.8% | 22.3% | (many diffs) |
| 6 | mouse | 171382 | Trpm8 | transient receptor potentia... | NM_134252.3 | 14.4% | 14.6% | (many diffs) |
| 7 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529225.1 | 14.4% | 14.6% | (many diffs) |
| 8 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529226.1 | 14.4% | 14.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa atccttcctt cctgtccaca ccatcgtgct tatcagggag aatgtgtgca 121 agtgtggcta tgcccagagc cagcacatgg aaggcaccca gatcaaccaa agtgagaaat 181 ggaactacaa gaaacacacc aaggaatttc ctaccgacgc ctttggggat attcagtttg 241 agacactggg gaagaaaggg aagtatatac gtctgtcctg cgacacggac gcggaaatcc 301 tttacgagct gctgacccag cactggcacc tgaaaacacc caacctggtc atttctgtga 361 ccgggggcgc caagaacttc gccctgaagc cgcgcatgcg caagatcttc agccggctca 421 tcTACATCGC GCAGTCCAAA GGTGCTTGGA TTCTCACGGG AGGCACCCAT TATGGCCTGA 481 TGAAGTACAT CGGGGAGGTG GTGAGAGATA ACACCATCAG CAGGAGTTCA GAGGAGAATA 541 TTGTGGCCAT TGGCATAGCA GCTTGGGGCA TGGTCTCCAA CCGGGACACC CTCATCAGGA 601 ATTGCGATGC TGAGGTACCG GTGGGACAGG AGGAGGTCTG CTACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAAAG AGCTGTGTTT CACCTTGACG CACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t