Construct: ORF TRCN0000470393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011714.1_s317c1
- Derived from:
- ccsbBroadEn_14017
- DNA Barcode:
- ACAGGTTTTCAGCGTGGGCTAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SELENOF (9403)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9403 | SELENOF | selenoprotein F | NM_004261.5 | 98.1% | 96.8% | 1_9delATGGTAGCG |
| 2 | human | 9403 | SELENOF | selenoprotein F | NM_203341.2 | 73.3% | 96.8% | 1_9delATGGTAGCG;312_313ins50;372_373ins73 |
| 3 | human | 9403 | SELENOF | selenoprotein F | NR_144513.1 | 28.2% | (many diffs) | |
| 4 | human | 9403 | SELENOF | selenoprotein F | NR_144512.1 | 28.2% | (many diffs) | |
| 5 | mouse | 93684 | Selenof | selenoprotein F | NM_053102.2 | 87.6% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 342
- ORF length:
- 276
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgggccg agtgggtgtc tggtgccGGC GTTTGGGCTA CGGTTGTTGT 121 TGGCGACTGT GCTTCAAGCG GTGTCTGCTT TTGGGGCAGA GTTTTCATCG GAGGCATGCA 181 GAGAGTTAGG CTTTTCTAGC AACTTGCTTT GCAGCTCTTG TGATCTTCTC GGACAGTTCA 241 ACCTGCTTCA GCTGGATCCT GATTGCAGAG GATGCTGTCA GGAGGAAGCA CAATTTGAAA 301 CCAAAAAGCT GTATGCAGGA GCTATTCTTG AAGTTTGTGG ATGAAAATTG GGAAGGTTCC 361 CTCAAGTCCA AGCTTTTGTT AGGAGTGATA AACCCAAACT GTTCAGAGGA CTGCAAATCA 421 AGTATGTCCG TGGTTCAGAC CCTGTATTAA AGCTTTTGGA CGACAATGGG AACATTGCTG 481 AAGAACTGAG CATTCTCAAA TGGAACACAG ACAGTGTAGA AGAATTCCTG AGTGAAAAGT 541 TGGAACGCAT ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA GGTTTTCAGC GTGGGCTAAT 721 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t