Construct: ORF TRCN0000470401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018879.3_s317c1
- Derived from:
- ccsbBroadEn_15238
- DNA Barcode:
- TTTTAACACTTCTATTATAGATAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- UHMK1 (127933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_175866.5 | 99.6% | 37.9% | (many diffs) |
| 2 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_144624.2 | 81.6% | 46.2% | (many diffs) |
| 3 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_001184763.1 | 80.5% | 17.1% | (many diffs) |
| 4 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | NM_010633.3 | 92.6% | 37.7% | (many diffs) |
| 5 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | XM_006496682.3 | 75.6% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 555
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggatcc ggctgcgcct ggggcgcgga gccgccgcgt tttctggagg 121 ccttcgggcg gctgtggcag gtacagagcc gtctgggtag cggctcctcc gcctcggtgt 181 atcgggttcg ctgctgcggc aaccctggct cgccccccgg cgccctcaag cagttcttgc 241 cgccaggaac caccggggct gcggcctctg ccgccgagta tggtttccgc aaagagaggg 301 cggcgctgga acagttgcag ggtcacagaa acatcgtgac tttgtatgga gtgtttacaa 361 tccacttttc tccaaatgtg ccatcacgct gtctgttgct tgaactcctg gatgtcagtg 421 tttcggaatt gctcttatat tccagtcacc agggttgttc catgtggatg atacagcatt 481 gtgcccgaga tgttttggag gcccttgctt ttcttcatca tgagggctat gtccatgcgg 541 acctccaanc cacgtaacat attgtggagt gcagagaatg aatgttttaa antcattgga 601 ctttggactt agcttcaaag aaggcaatca ggatgtaaag tatattcaga cagacgggta 661 tcgggctcca gaagcagaat tgcaaaattg cttggcccag gctggcctgc agagtgatac 721 agaatgtacc tcagctgttg atctgtggag cctaggaatc attttactgg aaatgttctc 781 aggaatgaaa ctgaaacata cagtcagatc tcaggaatgg aaggcaaaca gttctgctat 841 tattgatcac atatttgcca gtaaagcagt ggtgaatgcc gcaattccag cctatcacct 901 aagagacctt atcaaaagca tgcttcatga tgatccaagc agaagaatTC CTGCTGAAAT 961 GGCATTGTGC AGCCCATTCT TTAGCATTCC TTTTGCCCCT CATATTGAAG ATCTGGTCAT 1021 GCTTCCCACT CCAGTGCTAA GACTGCTGAA TGTGCTGGAT GATGATTATC TTGAGAATGA 1081 AGAGGAATAT GAAGATGTTG TAGAAGATGT AAAAGAGGAG TGTCAAAAAT ATGGACCAGT 1141 GGTATCTCTA CTTGTTCCAA AGGAAAATCC TGGCAGAGGA CAAGTCTTTG TTGAGTATGC 1201 AAATGCTGGT GATTCCAAAG CTGCGCAGAA ATTACTGACT GGAAGGATGT TTGATGGGAA 1261 GTTTGTTGTG GCTACATTCT ACCCGCTGAG TGCCTACAAG AGGGGATATC TGTATCAAAC 1321 CTTGCTTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 1381 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 1441 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTTTAAC ACTTCTATTA TAGATACACG 1501 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt