Construct: ORF TRCN0000470405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014545.1_s317c1
- Derived from:
- ccsbBroadEn_10783
- DNA Barcode:
- GCGACCGCACGGGATGCCATATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNA2 (1763)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1763 | DNA2 | DNA replication helicase/nu... | NM_001080449.3 | 64.8% | 62.8% | 1987_2101del;2177_3180del |
| 2 | human | 1763 | DNA2 | DNA replication helicase/nu... | XM_006717680.3 | 63% | 61% | 1_90del;2077_2191del;2267_3270del |
| 3 | human | 1763 | DNA2 | DNA replication helicase/nu... | NR_102264.1 | 49.6% | 1_89del;2151_4150del | |
| 4 | human | 1763 | DNA2 | DNA replication helicase/nu... | XM_017015799.1 | 43.6% | 41.7% | 0_1ins672;1315_1429del;1505_2508del |
| 5 | human | 1763 | DNA2 | DNA replication helicase/nu... | XM_011539417.1 | 30.8% | 28.9% | 0_1ins1080;907_1021del;1097_2100del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2130
- ORF length:
- 2061
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcagctg aacgaactgg agctgctgat ggagaagagt ttttgggagg 121 aggcggagct gccggcggag ctatttcaga agaaagtggt agcttccttt ccaagaacag 181 ttctgagcac aggaatggat aaccggtacc tggtgttggc agtcaatact gtacagaaca 241 aagagggaaa ctgtgaaaag cgcctggtca tcactgcttc acagtcacta gaaaataaag 301 aactatgcat ccttaggaat gactggtgtt ctgttccagt agagccagga gatatcattc 361 atttggaggg agactgcaca tctgacactt ggataataga taaagatttt ggatatttga 421 ttctgtatcc agacatgctg atttctggca ccagcatagc cagtagtatt cgatgtatga 481 gaagagctgt cctgagtgaa acttttagga gctctgatcc agccacacgc caaatgctaa 541 ttggtacggt tctccatgag gtgtttcaaa aagccataaa taatagcttt gccccagaaa 601 agctacaaga acttgctttt caaacaattc aagaaataag acatttgaag gaaatgtacc 661 gcttaaatct aagtcaagat gaaataaaac aagaagtaga ggactatctt ccttcgtttt 721 gtaaatgggc aggagatttc atgcataaaa acacttcgac tgacttccct cagatgcagc 781 tctctctgcc aagtgataat agtaaggata attcaacatg taacattgaa gtcgtgaaac 841 caatggatat tgaagaaagc atttggtccc ctaggtttgg attgaaaggc aaaatagatg 901 ttacagttgg tgtgaaaata catcgagggt ataaaacaaa atacaagata atgccgctgg 961 aacttaaaac tggcaaagaa tcaaattcta ttgaacaccg tagtcaggtt gttctgtaca 1021 ctctactaag ccaagagaga agagctgatc cagaggctgg cttgcttctc tacctcaaga 1081 ctggtcagat gtaccctgtg cctgccaacc atctagataa aagagaatta ttaaagctaa 1141 gaaaccagat ggcattctca ttgtttcacc gtattagcaa atctgctact agacagaaga 1201 cacagcttgc ttctttgcca caaataattg aggaagagaa aacttgtaaa tattgttcac 1261 aaattggcaa ttgtgctctt tatagcagag cagttgaaca acagatggat tgtagttcag 1321 tcccaattgt gatgctgccc aaaatagaag aagaaaccca gcatctgaag caaacacact 1381 tagaatattt cagcctttgg tgtctaatgt taaccctgga gtcacaatcg aaggataata 1441 aaaagaatca ccaaaatatc tggctaatgc ctgcttcgga aatggagaag agtggcagtt 1501 gcattggaaa cctgattaga atggaacatg taaagatagt ttgtgatggg caatatttac 1561 ataatttcca atgtaaacat ggtgccatac ctgtcacaaa tctaatggca ggtgacagag 1621 ttattgtaag tggagaagaa aggtcactgt ttgctttgtc tagaggatat gtgaaggaga 1681 ttaacatgac aacagtaact tgtttattag acagaaactt gtcggtcctt ccagaatcaa 1741 ctttgttcag attagaccaa gaagaaaaaa attgtgatat agatacccca ttaggaaaTC 1801 TTTCCAAATT GATGGAAAAC ACGTTTGTCA GCAAAAAACT TCGAGATTTA ATTATTGACT 1861 TTCGTGAACC TCAGTTTATA TCCTACCTTA GTTCTGTTCT TCCACATGAT GCAAAGGATA 1921 CAGTTGCCTG CATTCTAAAG GGTTTGAATA AGCCTCAGAG GCAAGCGATG AAAAAGGTAC 1981 TTCTTTCAAA AGACTACACA CTCATCGTGG GTATGCCTGG GACAGGAAAA ACAACTACGA 2041 TATGTACTCT CGTAAGATTC AGAAGGTTCA TCCAGCTATC CAGCAATTTA CAGAGCAAGA 2101 AATTTGCAGA TCAAAGTCCA TTAAATCCTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2161 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2221 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGAC 2281 CGCACGGGAT GCCATATGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2341 tgaaagatt