Construct: ORF TRCN0000470409
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004124.1_s317c1
- Derived from:
- ccsbBroadEn_13185
- DNA Barcode:
- GTGATCGAATCAATGCCCACTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MUCL3 (135656)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470409
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135656 | MUCL3 | mucin like 3 | NM_080870.4 | 16.8% | 16.8% | 1_3474del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac acaagtcaca gaaaagtcca cagaacaccc agaaaagacc acgtcaacca 121 cagagaaaac cacaagaacc ccagaaaagc ctacgctata ctcagagaag accatatgca 181 ccaaagggaa aaacacacca gtcccagaaa agcctacaga aaacctgggg aacaccacac 241 tgaccactga gaccataaaa gccccagtaa agtccacaga aaacccagaa aaaacagcag 301 cagtcacaaa gactataaaa ccttcagtca aggtcacagg agacaaatct ctcactacta 361 cctcttctca tctaaataaa actgaagtta ctcatcaggT GCCCACTGGT TCTTTCACCC 421 TCATTACATC TAGAACGAAG CTGAGTTCTA TCACATCAGA AGCCACAGGA AACGAGAGCC 481 ATCCATACCT CAATAAAGAT GGCTCACAGA AAGGTATCCA CGCTGGACAG ATGGGAGAGA 541 ATGATTCATT CCCTGCATGG GCCATAGTTA TTGTGGTCCT GGTGGCTGTG ATTCTCCTCC 601 TGGTGTTCCT TGGCCTGATC TTCTTGGTCT CCTATATGAT GCGGACACGC CGCACACTAA 661 CCCAGAACAC CCAGTACAAT GATGCAGAGG ATGAGGGTGG CCCCAATTCC TACCCGGTCT 721 ACCTGATGGA GCAGCAGAAT CTTGGCATGG GCCAGATCCC TTCCCCACGG TGCCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGAGTGA TCGAATCAAT GCCCACTCTA ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt