Construct: ORF TRCN0000470410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012531.1_s317c1
- Derived from:
- ccsbBroadEn_12383
- DNA Barcode:
- AAGTCCAACCTTAGTTTGTGGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYH7B (57644)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57644 | MYH7B | myosin heavy chain 7B | NM_020884.5 | 8.8% | 8.8% | 1_129del;654_5949delinsG |
| 2 | human | 57644 | MYH7B | myosin heavy chain 7B | XM_006723840.3 | 8.7% | 8.7% | 1_129del;654_5967delinsG |
| 3 | human | 57644 | MYH7B | myosin heavy chain 7B | XM_011528941.2 | 8.7% | 8.3% | 1_129del;626_686del;715_5991delinsG |
| 4 | human | 57644 | MYH7B | myosin heavy chain 7B | XM_017027986.1 | 8.7% | 8.7% | 1_129del;654_6009delinsG |
| 5 | mouse | 668940 | Myh7b | myosin, heavy chain 7B, car... | NM_001085378.2 | 7.8% | 8.2% | (many diffs) |
| 6 | mouse | 668940 | Myh7b | myosin, heavy chain 7B, car... | XM_017319211.1 | 7.8% | 8.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgtgagtgaa cttggggagt ctgcccgcta cctccgccag ggctaccagg 121 agatgacgaa ggtgcacact atcccatggg acgggaagaa gcgagtctgg gtgcctgatg 181 aacaggacgc cTACGTGGAG GCCGAGGTCA AGTCGGAGGC TACCGGGGGC AGAGTCACCG 241 TGGAGACCAA AGACCAGAAG GTGCTGATGG TGCGTGAAGC CGAGCTGCAG CCCATGAACC 301 CGCCTCGCTT CGACTTACTG GAGGACATGG CCATGATGAC GCACCTGAAC GAGGCCTCTG 361 TGCTGCACAA CCTGCGCCAG CGCTATGCCC GCTGGATGAT CTATACCTAC TCAGGCCTCT 421 TCTGTGTCAC CATCAACCCC TACAAATGGC TCCCAGTCTA TACGGCCTCC GTAGTGGCTG 481 CTTACAAGGG AAAGCGCCGC TCAGATTCCC CGCCCCATAT ATATGCGGTG GCGGACAACG 541 CCTACAACGA CATGCTGCGC AACCGAGACA ACCAGTCCAT GCTGATCACG TGCCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGAAAGT CCAACCTTAG TTTGTGGGTA ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt