Construct: ORF TRCN0000470417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010849.1_s317c1
- Derived from:
- ccsbBroadEn_12105
- DNA Barcode:
- TGATTTTGGGTGATACGGCTTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTMR10 (54893)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54893 | MTMR10 | myotubularin related protei... | XM_011521738.3 | 25.4% | 15.2% | (many diffs) |
| 2 | human | 54893 | MTMR10 | myotubularin related protei... | XM_011521737.3 | 25% | 15% | (many diffs) |
| 3 | human | 54893 | MTMR10 | myotubularin related protei... | XR_931859.3 | 23.4% | (many diffs) | |
| 4 | human | 54893 | MTMR10 | myotubularin related protei... | XM_017022384.2 | 23.3% | 14% | (many diffs) |
| 5 | human | 54893 | MTMR10 | myotubularin related protei... | NM_017762.3 | 17.4% | 10.6% | (many diffs) |
| 6 | mouse | 233315 | Mtmr10 | myotubularin related protei... | NM_172742.2 | 15.1% | 11% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc attacagaaa ttccattaca gaaaccttct tcttggtgaa cacgatgtcc 121 ctttaacatg tattgaacaa attgtcacag taaacgacca caagaggaag cagaaagtcc 181 TAGGCCCCAA CCAGAAACTG AAATTTAATC CAACAGAGTT AATTATTTAT TGTAAAGATT 241 TCAGAATTGT CAGATTTCGC TTTGATGAAT CAGGTCCCGA AAGTGCTAAA AAGCAAACAA 301 AATTAATGGA ATTCCCTCAG GAGATGGAGG AGGAGGAGGA GGAGGAGGTA ATGGAGCTGG 361 TGGTGGCAGC AGCCAGAAAA CTCCACTCTT TGAAACTTAC TCGGATTGGG ACAGAGAAAT 421 CAAGAGGACA GGTGCTTCCG GGTGGAGAGT TTGTTCTATT AACGAGGGTT ACATGCCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAT GATTTTGGGT GATACGGCTT CTAACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att