Construct: ORF TRCN0000470422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004963.1_s317c1
- Derived from:
- ccsbBroadEn_02085
- DNA Barcode:
- CATTGTTCCGCTCTCCCCGGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC22C (9117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_001201572.2 | 100% | 100% | |
| 2 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_004206.4 | 100% | 100% | |
| 3 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453814.1 | 100% | 100% | |
| 4 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453815.1 | 100% | 100% | |
| 5 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_001201584.2 | 91.2% | 91.2% | 642_643ins66 |
| 6 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | NM_032970.4 | 81.4% | 77.3% | (many diffs) |
| 7 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_011534212.2 | 81.4% | 77.3% | (many diffs) |
| 8 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453812.1 | 81.4% | 77.3% | (many diffs) |
| 9 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453813.1 | 81.4% | 77.3% | (many diffs) |
| 10 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_017007438.2 | 72% | 72% | 0_1ins210 |
| 11 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_017007439.2 | 72% | 72% | 0_1ins210 |
| 12 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_005265533.1 | 58.3% | 54.6% | (many diffs) |
| 13 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_005265534.1 | 58.3% | 54.6% | (many diffs) |
| 14 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_005265535.1 | 58.3% | 54.6% | (many diffs) |
| 15 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453816.1 | 58.3% | 54.6% | (many diffs) |
| 16 | human | 9117 | SEC22C | SEC22 homolog C, vesicle tr... | XM_024453817.1 | 58.3% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 816
- ORF length:
- 750
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgtgatcttt tttgcctgcg tggtacgggt aagggatgga ctgcccctct 121 cagcctctac tgatttttac cacacccaag attttttgga atggaggaga cggctcaaga 181 gtttagcctt gcgactggcc cagtatccag gtcgaggttc tgcagaaggt tgtgacttta 241 gtatacattt ttcttctttc ggggacgtgg cctgcatggc tatctgctcc tgccagtgtc 301 cagcagccat ggccttctgc ttcctggaga ccctgtggtg ggaattcaca gcttcctatg 361 acactaccTG CATTGGCCTA GCCTCCAGGC CATACGCTTT TCTTGAGTTT GACAGCATCA 421 TTCAGAAAGT GAAGTGGCAT TTTAACTATG TAAGTTCCTC TCAGATGGAG TGCAGCTTGG 481 AAAAAATTCA GGAGGAGCTC AAGTTGCAGC CTCCAGCGGT TCTCACTCTG GAGGACACAG 541 ATGTGGCAAA TGGGGTGATG AATGGTCACA CACCGATGCA CTTGGAGCCT GCTCCTAATT 601 TCCGAATGGA ACCAGTGACA GCCCTGGGTA TCCTCTCCCT CATTCTCAAC ATCATGTGTG 661 CTGCCCTGAA TCTCATTCGA GGAGTTCACC TTGCAGAACA TTCTTTACAG GTTGCCCATG 721 AGGAAATTGG AAACATTCTG GCTTTTCTTG TTCCTTTCGT AGCCTGCATT TTCCAGGATC 781 CAAGGAGCTG GTTCTGCTGG TTGGACCAAA CCTCGTGCCC AACTTTCTTG TACAAAGTGG 841 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 901 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 961 ACATTGTTCC GCTCTCCCCG GGCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1021 aatttgtgaa agatt