Construct: ORF TRCN0000470443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016020.1_s317c1
- Derived from:
- ccsbBroadEn_01882
- DNA Barcode:
- TCAACTGCCCGGGCTAAAATCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERF1A (8293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8293 | SERF1A | small EDRK-rich factor 1A | NM_021967.2 | 100% | 100% | |
| 2 | human | 728492 | SERF1B | small EDRK-rich factor 1B | NM_022978.3 | 100% | 100% | |
| 3 | human | 728492 | SERF1B | small EDRK-rich factor 1B | NM_001178087.1 | 45.2% | 38.1% | (many diffs) |
| 4 | human | 8293 | SERF1A | small EDRK-rich factor 1A | NM_022968.2 | 45.2% | 38.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC CCGTGGAAAT CAACGAGAAC TTGCCCGCCA GAAAAACATG AAGAAAACCC 121 AGGAAATTAG CAAGGGAAAG AGGAAAGAGG ATAGCTTGAC TGCCTCTCAG AGAAAGCAGA 181 GTTCTGGAGG CCAGAAATCT GAGAGCAAGA TGTCAGCTGG GCCACACCTC CCTCTGAAGG 241 CTCCAAGGGA GAATCCTTGC TTTCCTCTTC CAGCTGCTGG TGGCTCCAGG TATTACTTGG 301 CTTATGGCAG CATAACTCCT ATCTCTGCCT TTGTCTTTGT GGTCTTCTTT TCTGTCTTCT 361 TCCCTTCTTT TTATGAGGAC TTTTGCTGTT GGATTTACCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ATCAACTGCC CGGGCTAAAA TCAGCACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt