Construct: ORF TRCN0000470470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003669.1_s317c1
- Derived from:
- ccsbBroadEn_04352
- DNA Barcode:
- CCCGAATCGCAACTCCATCCGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHCHD5 (84269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | NM_032309.4 | 100% | 100% | |
| 2 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | XM_005263824.4 | 68.4% | 65.8% | (many diffs) |
| 3 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | NM_001304353.2 | 65.4% | 65.4% | 0_1ins114 |
| 4 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | NM_001304354.2 | 65.4% | 65.4% | 0_1ins114 |
| 5 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | NM_001371322.1 | 65.4% | 65.4% | 0_1ins114 |
| 6 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | NM_001371323.1 | 65.4% | 65.4% | 0_1ins114 |
| 7 | human | 84269 | CHCHD5 | coiled-coil-helix-coiled-co... | NR_163920.1 | 61.8% | (many diffs) | |
| 8 | mouse | 66170 | Chchd5 | coiled-coil-helix-coiled-co... | NM_025395.3 | 85.7% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCA GGCGGCCCTA GAGGTCACCG CTCGCTACTG TGGCCGGGAG CTGGAGCAGT 121 ATGGCCAGTG TGTGGCGGCC AAGCCGGAAT CCTGGCAGCG GGACTGTCAC TACCTTAAGA 181 TGAGCATTGC CCAGTGCACA TCCTCCCACC CAATCATCCG CCAGATCCGC CAGGCCTGTG 241 CTCAGCCTTT TGAGGCCTTC GAGGAGTGTC TTCGACAGAA CGAGGCAGCT GTGGGCAACT 301 GTGCAGAGCA TATGCGCCGC TTCCTGCAGT GCGCTGAGCA GGTGCAGCCG CCACGCTCAC 361 CTGCAACTGT GGAGGCACAG CCACTTCCTG CCTCCTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ACCCGAATCG CAACTCCATC CGTGAACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt