Construct: ORF TRCN0000470471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014374.1_s317c1
- Derived from:
- ccsbBroadEn_12882
- DNA Barcode:
- GTCCGCCCCAAAGCGACTCGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM104A (84923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84923 | FAM104A | family with sequence simila... | XM_024451008.1 | 81.7% | 80.1% | (many diffs) |
| 2 | human | 84923 | FAM104A | family with sequence simila... | NM_001289412.1 | 81.3% | 80.8% | (many diffs) |
| 3 | human | 84923 | FAM104A | family with sequence simila... | NM_032837.2 | 64.3% | 64.5% | 1_198del;375T>C |
| 4 | human | 84923 | FAM104A | family with sequence simila... | NM_001098832.1 | 57.8% | 57.9% | 1_198del;321_383del;438T>C |
| 5 | mouse | 28081 | Fam104a | family with sequence simila... | NM_138598.5 | 54.3% | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgagcgcctc cgtcccagga aaaggagaag gaatggcaac gaagaagaca 121 accatcttCC CCCCCAGACC AAAAGAAGTA GCAGAAACCC TGTCTTTCAG GATTCCTGGG 181 ACACAGAGTC TTCAGGCAGT GACAGTGGTG GGAGCAGCAG CAGCAGCAGC AGCAGCATCA 241 ACAGCCCGGA CAGGGCCAGC GGGCCGGAAG GCAGCTTGAG CCAGACCATG GCCGGATCCA 301 GCCCTAACAC GCCTCAGCCC GTGCCCGAGC AGTCCGCGCT GTGCCAAGGC CTCTACTTCC 361 ACATCAACCA GACCCTGAGG GAGGCCCACT TCCACAGCCT ACAGCACCGA GGGCGGCCTC 421 TGACATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTCCGCCCC AAAGCGACTC GAATTACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt