Construct: ORF TRCN0000470517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017495.1_s317c1
- Derived from:
- ccsbBroadEn_04784
- DNA Barcode:
- GTCGGATTACGACGGGACCCCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX20 (124460)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124460 | SNX20 | sorting nexin 20 | NM_153337.3 | 100% | 100% | |
| 2 | human | 124460 | SNX20 | sorting nexin 20 | NM_001144972.2 | 77.7% | 75.5% | (many diffs) |
| 3 | human | 124460 | SNX20 | sorting nexin 20 | NM_182854.4 | 37.7% | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC AAGTCCAGAG CACCCTGGGA GCCCTGGCTG CATGGGACCC ATAACCCAGT 121 GCACGGCAAG GACCCAGCAG GAAGCACCAG CCACTGGCCC CGACCTCCCG CACCCAGGAC 181 CTGACGGGCA CTTAGACACA CACAGTGGCC TGAGCTCCAA CTCCAGCATG ACCACGCGGG 241 AGCTTCAGCA GTACTGGCAG AACCAGAAAT GCCGCTGGAA GCACGTCAAA CTGCTCTTTG 301 AGATCGCTTC AGCTCGCATC GAGGAGAGAA AAGTCTCTAA GTTTGTGATG GGGAAATCAA 361 GGCCTGGAGA GATGACTTAT CCAGGGTCAC GTGGCGAGAC AGGGACAGCA CCAGAACCAG 421 ACCCGAGATG TCCACGTCAA AGTGACATGC TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 601 CGGATTACGA CGGGACCCCG ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt