Construct: ORF TRCN0000470523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012658.1_s317c1
- Derived from:
- ccsbBroadEn_14423
- DNA Barcode:
- GATCGAGGCGAACCATGAATCAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TENT5D (169966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169966 | TENT5D | terminal nucleotidyltransfe... | NM_001170574.1 | 99.6% | 98.7% | (many diffs) |
| 2 | human | 169966 | TENT5D | terminal nucleotidyltransfe... | NM_152630.4 | 99.6% | 98.7% | (many diffs) |
| 3 | human | 169966 | TENT5D | terminal nucleotidyltransfe... | XM_024452344.1 | 99.6% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgaaatcaga ttcaccaatc tcgcttggga tcaagttata acactggatc 121 aagtgttaga tgaagtaatt ccaattcatg gaaaggggaa tttccccaca atggaggtaa 181 aaccaaaaga catcattcat gttgtgaaag atcaactcat agggcaagga attattgtta 241 aagatgccag attgaatggt tccgtagcaa gttacatact tgcaagccac aatggaatca 301 gctataagga tctggacgtt atttttggtg ttgagcttcc aggtaacgaa gaatttcagg 361 ttgttaaaga tgcagttcta gactgtctac ttgacttttt accaaaagat gtaaagaagg 421 aaaagctctc cccagatatc atgaaagacg cttacgtaca gaaattggtc aaggtttgca 481 atgggcatga ttgttggagt cttatctccc tttcaaataa cactgggaag aatttagaac 541 taaaatttgt gagttcactc agacggcagt ttgaatttag tgtagattcc tttcaaattg 601 ttttggatcc catgttagac ttctacagtg acaaaaatgc caagctaacc aaagaatcct 661 atcctgttgt ggtagctgaa agcatgtatg gagacttcca ggaagcaatg acacatttgc 721 aacacaagct catatgtacc aggaaacctg aagagattag aggtggtggc cttctgaagt 781 actgcagctt gctggttcat ggcttcaagc cagcctgtat gtcagaaatc aaaaacctag 841 aacgttatat gtgctctaga ttctttattg attttcctca tatagaagaa cagcaaaaga 901 aaattgaatc atacctccac aaccatttca taggtgaagg aatgaccaag tatgactacc 961 ttatgacctt gcatggagtt gtgaatgaaa gcactgtttg cctcatgagt tatgaaagaa 1021 gacagattct ccacctgatc accatgatgg ctttgaaagt acttggagaa ctaaatattc 1081 tacccaatac acaaaaggta acttGCTTTT ATCAGCCTGC TCCGTACTTT GCAGCTGAGG 1141 CAAGGTACCC TATTTATGTA ATACCTGAGC CACCCCCCGT TAGCTTCCAG CCATACCACC 1201 CACTGCACCT TCGTGTATCA AATGGTTGAG TTACCCAACT TTCTTGTACA AAGTGGTTGA 1261 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1321 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAT 1381 CGAGGCGAAC CATGAATCAG CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1441 tgtgaaagat t