Construct: ORF TRCN0000470561
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001385.1_s317c1
- Derived from:
- ccsbBroadEn_00449
- DNA Barcode:
- TGTTTGGTCTGATGTGTGTGATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLX4 (1748)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470561
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1748 | DLX4 | distal-less homeobox 4 | NM_138281.3 | 100% | 100% | |
| 2 | human | 1748 | DLX4 | distal-less homeobox 4 | NM_001934.3 | 68% | 63.3% | (many diffs) |
| 3 | human | 1748 | DLX4 | distal-less homeobox 4 | XM_017024291.1 | 68% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 786
- ORF length:
- 720
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ctctttgccc tgccccctcc ccggccggga cgcctccaaa gctgtcttcc 121 cagacctcgc ccctgtcccg tcggtagcgg ctgcctaccc gcttggcttg tcccctacaa 181 ccgcagcctc ccccaatttg tcctactcca ggccgtatgg ccacctcctg tcttacccct 241 acaccgagcc agcgaacccc ggagactcct acctgtcctg ccagcaaccc gcggcgctct 301 ctcagcccct ctgcggacct gcagagcacc ctcaggaact cgaggcagac tcggagaagc 361 cgcggctgtc cccggaaccc tccgagcggc gccctcaggc ccccgccaaa aagctccgca 421 agccgaggac catctactcc agcctgcagc tgcagcacct aaaccagcgt ttccagcaca 481 cgcagtacct ggcgctgccc gagagggccc agctggcagc gcagctcggc ctcacccaga 541 cccaggtaaa gatctggttt cagaacaaac gctccaagta taagaagctc ctgaagcaga 601 attctggggg gcaggaaggg gacttccctg ggaggacctt ctcTGTGTCT CCCTGCTCCC 661 CACCCCTCCC CTCCCTCTGG GATCTACCCA AGGCAGGGAC CCTGCCCACC AGTGGCTATG 721 GCAACAGCTT TGGAGCCTGG TATCAGCATC ACTCCTCAGA TGTCCTGGCT TCGCCTCAGA 781 TGATGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 841 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 901 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGTTTGGTC TGATGTGTGT GATAAACGCG 961 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt