Construct: ORF TRCN0000470581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003373.1_s317c1
- Derived from:
- ccsbBroadEn_08330
- DNA Barcode:
- GATCAGCATAGCTTTATCCCCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC2LI1 (51626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_015522.3 | 99.6% | 99% | 56T>C;596G>C |
| 2 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_016008.4 | 53.7% | 49.1% | (many diffs) |
| 3 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001193464.2 | 53.4% | 49% | (many diffs) |
| 4 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001348912.1 | 50.5% | 46.2% | (many diffs) |
| 5 | human | 51626 | DYNC2LI1 | dynein cytoplasmic 2 light ... | NM_001348913.1 | 50.3% | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cagtgaaact ctctgggaaa ttgcaaaagc tgaagtggaa aaaaggggaa 121 ctaatggaag tgaaggtgat ggagctgaaa ttgcagaaaa atttgttttc ttcattggca 181 gtaaaaatgg gggaaagact actattattc taaggtgtct tgacagagat gaaccaccaa 241 aaccaaccTT AGCTTTGGAA TATACATATG GAAGAAGAGC AAAAGGGCAC AACACACCAA 301 AAGATATCGC TCACTTTTGG GAACTCGGTG GAGGAACCTC TTTATTGGAC TTAATCAGCA 361 TACCCATCAC AGGTGACACC TTACGGACGT TTTCTCTTGT TCTCGTTCTG GATCTTTCAA 421 AACCTAATGA TCTCTGGCCC ACCATGGAAA ATCTCTTGCA AGCCACAAAA AGCCATGTAG 481 ACAAAGTGAT AATGAAACTG GGAAAGACAA ATGCTAAAGC AGTTTCTGAA ATGAGACAGA 541 AGATCTGGAA TAATATGCCG AAGGATCATC CTGTGAGTTG CTGTTTGGGA TTATTACTGG 601 AATCCTTAGT CCCATTTATA GTTAATGATA ACATCACAAA CAACTTCTTT AGATTTTTAT 661 CCATGACTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGATCAG CATAGCTTTA TCCCCTAAAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt