Construct: ORF TRCN0000470592
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015176.1_s317c1
- Derived from:
- ccsbBroadEn_12831
- DNA Barcode:
- TAAGCCGGAGTGCAACAAAGGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A8 (84561)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470592
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84561 | SLC12A8 | solute carrier family 12 me... | NM_001195483.1 | 43% | 42.9% | 1_1218del;1991G>A;1998T>C |
| 2 | human | 84561 | SLC12A8 | solute carrier family 12 me... | NM_024628.5 | 43% | 42.9% | 1_1218del;1991G>A;1998T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 990
- ORF length:
- 924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ttcctgcagc ctgaccccgg tgcctgagcc ggtgctcagg gagggcgcag 121 aaggcctcca ctgctctgag cacctgctct tagagaaagc tcccagttac ggctctgagg 181 gacctgccca aagagtcttg gagggcacgc tactggaatt caccaaggac atggatcagc 241 tcctccagct aaccaggaag cttgagagta gccagcccag gcaaggagag ggtaacagga 301 ccccagaaag tcagaagagg aaaagcaaga aggccaccaa gcagacccta caagatagct 361 tcctcttgga cctcaaatcc cctccttctt tccctgtcga gatctctgac aggttgcccg 421 ctgcctcctg ggaggggcag gagtcctgct ggaacaagca gacttccaag agcgaaggga 481 ctcagcctga gggaacatat ggagagcaac ttgttcctga gctgtgcaac caatcagagt 541 ccagtggaga agatttcttc ctGAAGTCCA GGCTCCAAGA ACAAGATGTC TGGAGAAGAT 601 CCACTTCTTT CTATACCCAC ATGTGCAACC CCTGGGTCTC CCTGTTGGGG GCTGTTGGGT 661 CCCTTCTCAT CATGTTTGTG ATACAGTGGG TGTATACCCT GGTTAACATG GGTGTTGCTG 721 CCATCGTGTA TTTCTACATT GGCCGGGCCA GTCCAGGGCT TCACCTTGGA TCAGCCTCCA 781 ACTTCAGCTT TTTCCGGTGG ATGAGGTCTC TCTTGCTCCC CTCCTGCAGG AGCTTGCAGT 841 CCCCCCAGGA GCAGATCATC TTGGCGCCGT CCCTGGCTAA GGTTGACATG GAGATGACTC 901 AGCTCACCCA GGAGAATGCA GACTTCGCCA CTCGGGATCG CTACCACCAC TCCTCCCTCG 961 TGAACCGGGA GCAGCTGATG CCTCACTACT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1021 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1081 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAAGC 1141 CGGAGTGCAA CAAAGGACTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1201 tgaaagatt