Construct: ORF TRCN0000470623
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006402.1_s317c1
- Derived from:
- ccsbBroadEn_12407
- DNA Barcode:
- TCCTTAACCTAGCATTCGGAACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC181 (57821)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470623
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57821 | CCDC181 | coiled-coil domain containi... | NM_001300968.1 | 73% | 72.6% | 515T>C;1099_1100insA;1116_1524del |
| 2 | human | 57821 | CCDC181 | coiled-coil domain containi... | NM_021179.2 | 73% | 72.6% | 515T>C;1099_1100insA;1116_1524del |
| 3 | human | 57821 | CCDC181 | coiled-coil domain containi... | XM_017001940.1 | 73% | 72.6% | 515T>C;1099_1100insA;1116_1524del |
| 4 | human | 57821 | CCDC181 | coiled-coil domain containi... | NM_001300969.1 | 72.9% | 72.5% | 515T>C;1099_1100insA;1116_1527del |
| 5 | human | 57821 | CCDC181 | coiled-coil domain containi... | XM_005245383.1 | 72.9% | 72.5% | 515T>C;1099_1100insA;1116_1527del |
| 6 | human | 57821 | CCDC181 | coiled-coil domain containi... | XM_011509827.2 | 72.9% | 72.5% | 515T>C;1099_1100insA;1116_1527del |
| 7 | human | 57821 | CCDC181 | coiled-coil domain containi... | XM_017001938.1 | 72.9% | 72.5% | 515T>C;1099_1100insA;1116_1527del |
| 8 | human | 57821 | CCDC181 | coiled-coil domain containi... | XM_017001939.1 | 72.9% | 72.5% | 515T>C;1099_1100insA;1116_1527del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgaaaataaa gatactgatt caaagaaaag tgaagaatac gaagatgact 121 ttgaaaagga cctggagtgg ttaattaatg aaaatgaaaa aagtgatgcc agcataatag 181 agatggcttg tgagaaggaa gagaatatta accaagactt aaaagagaat gagacagtaa 241 tggagcacac caaacggcat tctgatcctg acaaatcttt gcaggatgag gtctcaccaa 301 gaagaaatga catcatttct gtaccaggta ttcaaccttt ggatcccata tcagattcag 361 atagtgaaaa ctctttccag gaatccaaac tagaaagcca gaaagacttg gaggaggaag 421 aggatgagga agtaaggaga tatattatgg agaaaattgt acaagctaac aagcttctac 481 agaatcaaga accggtgaat gataaaaggg agcgaaaact taagttcaag gaccagttag 541 ttgatttgga agttcctcca ctagaagaca ctactacttc taaaaattat tttgaaaacg 601 aaaggaatat gtttgggaaa ctgtcacaat tatgtatttc caatgatttt ggacaagaag 661 atgtgctcct gtcacttact aatggaagct gtgaagaaaa caaggatagg acaatactgg 721 tagagagaga tggaaaattt GAACTTCTGA ATTTACAAGA CATTGCCAGT CAGGGGTTTT 781 TGCCTCCCAT TAATAATGCA AATAGTACAG AAAATGACCC TCAGCAGTTG TTACCCAGAT 841 CTTCCAACTC CTCTGTCAGT GGCACCAAGA AAGAAGATTC TACAGCAAAG ATTCATGCTG 901 TCACTCACTC ATCAACAGGA GAGCCGCTGG CTTATATCGC TCAGCCACCA CTCAACCGCA 961 AGACTTGTCC AAGCTCTGCT GTCAACTCAG ATCGAAGTAA AGGGAATGGG AAATCTAATC 1021 ACAGGACACA GTCTGCACAT ATCTCACCAG TGACTTCAAC ATACTGTCTT TCCCCTCGAC 1081 AGAAAGAACT ACAAAAACAA CTAGAAGAAA AGAGAGAAAA ACTGAAAAGA GAGGAAGAGC 1141 GACGAAAAAT AGAAGAAGAG AAAGAAAAAA AAGAGAGAGA ATGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGATCC TTAACCTAGC ATTCGGAACT GACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t