Construct: ORF TRCN0000470630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015607.1_s317c1
- Derived from:
- ccsbBroadEn_00641
- DNA Barcode:
- TATACCCCATATGAAACGGAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GJA5 (2702)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470630
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2702 | GJA5 | gap junction protein alpha 5 | NM_005266.6 | 100% | 100% | |
| 2 | human | 2702 | GJA5 | gap junction protein alpha 5 | NM_181703.4 | 100% | 100% | |
| 3 | human | 2702 | GJA5 | gap junction protein alpha 5 | XM_005272951.4 | 100% | 100% | |
| 4 | human | 2702 | GJA5 | gap junction protein alpha 5 | XM_017001044.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1140
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgattggagc ttcctgggaa atttcctgga ggaagtacac aagcactcga 121 ccgtggtagg caaggtctgg ctcactgtcc tcttcatatt ccgtatgctc gtgctgggca 181 cagctgctga gtcttcctgg ggggatgagc aggctgattt ccggtgtgat acgattcagc 241 ctggctgcca gaatgtctgc tacgaccagg ctttccccat ctcccacatt cgctactggg 301 tgctgcagat catcttcgtc tccacgccct ctctggtgta catgggccac gccatgcaca 361 ctgtgcgcat gcaggagaag cgcaagctac gggaggccga gagggccaaa gaggtccggg 421 gctctggctc ttacgagtac ccggtggcag agaaggcaga actgtcctgc tgggaggaag 481 ggaatggaag gattgccctc cagggcactc tgctcaacac ctatgtgtgc agcatcctga 541 tccgcaccac catggaggtg ggcttcattg tgggccagta cttcatctac ggaatcttcc 601 tgaccaccct gcatgtctgc cgcaggagtc cctgtcccca cccggtcaac tgttacgtat 661 cccggcccac agagaagaat gtcttcattg tctttatgct ggctgtggct gcactgtccc 721 tccTCCTTAG CCTGGCTGAA CTCTACCACC TGGGCTGGAA GAAGATCAGA CAGCGATTTG 781 TCAAACCGCG GCAGCACATG GCTAAGTGCC AGCTTTCTGG CCCCTCTGTG GGCATAGTCC 841 AGAGCTGCAC ACCACCCCCC GACTTTAATC AGTGCCTGGA GAATGGCCCT GGGGGAAAAT 901 TCTTCAATCC CTTCAGCAAT AATATGGCCT CCCAACAAAA CACAGACAAC CTGGTCACCG 961 AGCAAGTACG AGGTCAGGAG CAGACTCCTG GGGAAGGTTT CATCCAGGTT CGTTATGGCC 1021 AGAAGCCTGA GGTGCCCAAT GGAGTCTCAC CAGGTCACCG CCTTCCCCAT GGCTATCATA 1081 GTGACAAGCG ACGTCTTAGT AAGGCCAGCA GCAAGGCAAG GTCAGATGAC CTATCAGTGT 1141 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGATATAC CCCATATGAA ACGGAAATCA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt