Construct: ORF TRCN0000470640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001094.1_s317c1
- Derived from:
- ccsbBroadEn_03208
- DNA Barcode:
- ATCTGATAACATCCGTCACTGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APH1A (51107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NM_016022.3 | 100% | 100% | |
| 2 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NM_001077628.3 | 92.9% | 92.4% | 735_745delinsTA;751_795del |
| 3 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | XM_024447455.1 | 77.9% | 77.5% | 479_631del;888_898delinsTA;904_948del |
| 4 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NM_001243771.1 | 76.9% | 76.9% | 113_114ins171 |
| 5 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | XM_017001417.2 | 71.4% | 70.9% | 113_114ins171;564_574delinsTA;580_624del |
| 6 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NM_001243772.1 | 66.5% | 63% | (many diffs) |
| 7 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NR_045035.1 | 29.1% | 1_399del;683_684ins74;1067_2216del | |
| 8 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NR_045033.1 | 26.4% | 1_399del;511_512ins136;1005_2154del | |
| 9 | human | 51107 | APH1A | aph-1 homolog A, gamma-secr... | NR_045034.1 | 21.6% | 1_399del;511_512ins245;896_2045del | |
| 10 | mouse | 226548 | Aph1a | aph1 homolog A, gamma secre... | NM_146104.3 | 94.6% | 99.1% | (many diffs) |
| 11 | mouse | 226548 | Aph1a | aph1 homolog A, gamma secre... | NM_146134.2 | 87.9% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggctgcggtg tttttcggct gcactttcgt cgcgttcggc ccggccttcg 121 cgcttttctt gatcactgtg gctggggacc cgcttcgcgt tatcatcctg gtcgcagggg 181 catttttctg gctggtctcc ctgctcctgg cctctgtggt ctggttcatc ttggtccatg 241 tgaccgaccg gtcagatgcc cggctccagt acggcctcct gatttttggt gctgctgtct 301 ctgtccttct acaggaggtg ttccgctttg cctactacaa gctgcttaag aaggcagatg 361 aggggttagc atcgctgagt gaggacggaa gaTCACCCAT CTCCATCCGC CAGATGGCCT 421 ATGTTTCTGG TCTCTCCTTC GGTATCATCA GTGGTGTCTT CTCTGTTATC AATATTTTGG 481 CTGATGCACT TGGGCCAGGT GTGGTTGGGA TCCATGGAGA CTCACCCTAT TACTTCCTGA 541 CTTCAGCCTT TCTGACAGCA GCCATTATCC TGCTCCATAC CTTTTGGGGA GTTGTGTTCT 601 TTGATGCCTG TGAGAGGAGA CGGTACTGGG CTTTGGGCCT GGTGGTTGGG AGTCACCTAC 661 TGACATCGGG ACTGACATTC CTGAACCCCT GGTATGAGGC CAGCCTGCTG CCCATCTATG 721 CAGTCACTGT TTCCATGGGG CTCTGGGCCT TCATCACAGC TGGAGGGTCC CTCCGAAGTA 781 TTCAGCGCAG CCTCTTGTGT AAGGACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCTGATA 961 ACATCCGTCA CTGCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt