Construct: ORF TRCN0000470653
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005462.1_s317c1
- Derived from:
- ccsbBroadEn_10993
- DNA Barcode:
- GTTCATTCAGCCAGTCCCTTCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEFL (4747)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470653
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4747 | NEFL | neurofilament light | NM_006158.4 | 52.2% | 51.9% | (many diffs) |
| 2 | mouse | 18039 | Nefl | neurofilament, light polype... | NM_010910.1 | 47.3% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttccttcagc tacgagccgt actactcgac ctcctacaag cggcgctacg 121 tggagacgcc ccgggtgcac atctccagcg tgcgcagcgg ctacagcacc gcacgctcag 181 cttactccag ctactcggcg ccggtgtctt cctcgctgtc cgtgcgccgc agctactcct 241 ccagctctgg atcgttgatg cccagtctgg agaacctcga cctgagccag gtagccgcca 301 tcagcaacga cctcaagtcc atccgcacgc aggagaaggc gcagctccag gacctcaatg 361 accgcttcgc cagcttcatc gagcgcgtgc acgagctgga gcagcagaac aaggtcctgg 421 aagccgagct gctggtgctg cgccagaagc actccgagcc aTCCCGCTTC CGGGCGCTGT 481 ACGAGCAGGA GATCCGCGAC CTGCGCCTGG CGGCGGAAGA TGCCACCAAC GAGAAGCAGG 541 CGCTCCAGGG CGAGCGCGAA GGGCTGGAGG AGACCCTGCG CAACCTGCAG GCGCGCTATG 601 AAGAGGAGGT GCTGAGCCGC GAGGACGCCG AGGGCCGGCT GATGGAAGCG CGCAAAGGCG 661 CCAAGAACAC CGACGCCGTG CGCGCCGCCA AGGACGAGGT GTCCGAGAGC CGTCGTCTGC 721 TCAAGGCCAA GACCCTGGAA ATCGAAGCAT GCCGGGGCAT GAATGAAGCG CTGGAGAAGC 781 AGCTGCAGGA GCTGGAGGAC AAGCAGAACG CCGACATCAG CGTTATGCAG GACACGATCA 841 ACAAATTAGA AAATGAATTG AGGACCACAA AGAGTGAAAT GGCACGATAC CTAAAAGAAT 901 ACCAAGACCT CCTAACGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTCATT CAGCCAGTCC 1081 CTTCGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt