Construct: ORF TRCN0000470655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011070.1_s317c1
- Derived from:
- ccsbBroadEn_16129
- DNA Barcode:
- AATGAGGATATGAGTTTAGCAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH3 (221120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470655
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221120 | ALKBH3 | alkB homolog 3, alpha-ketog... | NM_139178.4 | 48.3% | 48.6% | 1_441del;726G>T;852A>G |
| 2 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | XM_017319248.1 | 71.4% | 76.7% | (many diffs) |
| 3 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | NM_026944.1 | 42% | 45.1% | (many diffs) |
| 4 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | XM_006500123.3 | 42% | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA ACCAAATCCT CACTGGCACC CTGTGCTGCG CACACTAAAG AACCGCATTG 121 AAGAGAACAC TGGCCACACC TTCAACTCCT TACTCTGCAA TCTTTATCGC AATGAGAAGG 181 ACAGCGTGGA CTGGCACAGT GATGATGAAC CCTCACTAGG GAGGTGCCCC ATTATTGCTT 241 CACTAAGTTT TGGTGCCACA CGCACATTTG AGATGAGAAA GAAGCCACCA CCAGAAGAGA 301 ATGGAGACTA CACATATGTG GAAAGAGTGA AGATACCCTT GGATCATGGT ACCTTGTTAA 361 TCATGGAAGG AGCGACACAA GCTGACTGGC AGCATCGAGT GCCCAAAGAA TACCACTCTA 421 GAGAACCGAG AGTGAACCTG ACCTTTCGGA CAGTCTATCC AGACCCTCGA GGGGCGCCCT 481 GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GCCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TGAGGATATG AGTTTAGCAG AAACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt