Construct: ORF TRCN0000470661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011494.1_s317c1
- Derived from:
- ccsbBroadEn_09699
- DNA Barcode:
- CTCAATTCCATACTTAGCTGCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC112 (153733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153733 | CCDC112 | coiled-coil domain containi... | NM_152549.2 | 99.8% | 99.5% | 1023G>T;1061A>G |
| 2 | human | 153733 | CCDC112 | coiled-coil domain containi... | NM_001040440.3 | 84.1% | 83.9% | 1_249del;1272G>T;1310A>G |
| 3 | human | 153733 | CCDC112 | coiled-coil domain containi... | XM_005271908.1 | 78.1% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaaagataaa cacagtcatt tctacaacca aaaaagtgac ttcagaattg 121 agcatagtat gctagaagaa ttggaaaata aattgattca cagcaggaaa acagaaagag 181 caaaaatcca gcaacaattg gccaaaatac ataataatgt aaagaaactt cagcatcaat 241 taaaagatgt gaagcctaca cctgattttg ttgagaagct cagagaaatg atggaagaaa 301 ttgaaaatgc aattaacact tttaaagaag agcagaggtt gatatatgaa gagctaatta 361 aagaagagaa gacaactaat aatgagttga gtgccatatc aagaaaaatt gacacatggg 421 ctttgggtaa ttcagaaaca gagaaagctt tcagagcaat ctcaagcaaa gttcctgtag 481 acaaagtaac accaagtact cttccagaag aggtactaga ttttgaaaaa ttccttcagc 541 aaacaggagg gcgacaaggt gcctgggatg attatgatca ccagaacttt gtaaaggtga 601 gaaacaaaca taaagggaag ccaacattta tggaagaagt tctagaacac cttcctggaa 661 aaacacaaga tgaagttcaa cagcatgaaa aatggtatca aaagtttctg gctctagaag 721 aaagaaaaaa agagtcaatt cagatttgga aaactaaaaa gcagcaaaaa agggaggaaa 781 ttttcaagtt aaaggaaaag gcagacaaca cacctgtgct ttttcataat aaacaagagg 841 ataatcaaaa gcaaaaagag gaacaaagaa agaaacagaa attggcagtt gaagcttgga 901 agaaacagaa aagtatagaa atGTCAATGA AATGTGCTTC CCAGTTAAAA GAAGAAGAAG 961 AGAAAGAGAA AAAACATCAG AAAGAACGCC AGCGCCAGTT TAAGTTAAAA TTACTACTAG 1021 AAAGTTATAC CCAGCAGAAG AAAGAACAGG AAGAATTTTT GAGGCTTGAA AAGGAGATAA 1081 GGGAAAATGC AGAAAAGGCA GAAAAAAGGA AAAATGCTGC TGATGGAATT TCCAGATTTC 1141 AAGAAAGAGA TTTACATAAA CTTGAACTGA AAATTCTAGA TAGACAGGCA AAGGAAGATG 1201 AAAAGTCACA AAAACAAAGA AGACTGGCAA AATTAAAAGA AAAGGTTGAA AACAATGTTA 1261 GTAGAGATCC CTCTAGGCTT TACAAACCCA CCAAAGGTTG GGAAGAACGA ACCAAAAAGA 1321 TAGGACCAAC AGGCTCTGGG CCACTTCTAC ATATCCCACA TAGGGCTATT CCAACCTGGA 1381 GACAAGGAAT ACAGAGAAGA GTATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TCAATTCCAT 1561 ACTTAGCTGC AACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att